A thin‐layer multistrip polyacrylamide gel electrophoresis apparatus for ferguson plot analysis at the sub‐microgram load level

László Orbán, James V. Sullivan, Christian Zwieb, Andreas Chrambach

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

A procedure was developed for casting thin‐layer multistrip polyacrylamide gels and using them for the simultaneous gel electrophoresis at several gel concentrations (Ferguson plot analysis) at the sub‐microgram load level, using silver staining, autoradiography and, potentially, blotting for detection. The lower viscosity of polymerization mixtures, compared to agarose gelation mixtures, required the redesign of the multistrip cassette with separation of channels by rubber gaskets and the application of a cassette press. The lowered viscosity also required addition of 35% sucrose and an increased rate of polymerization in application to multistrip gels formed on a common NetFix backing. The present design allows one to obtain Ferguson plots exemplified by those of 32P‐labeled DNA followed by autoradiographic detection.

Original languageEnglish (US)
Pages (from-to)726-729
Number of pages4
JournalELECTROPHORESIS
Volume10
Issue number10
DOIs
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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