Abstract
A technique has been developed for embedding several agarose gels (running gels), each of a different agarose concentration, within a single 1.5% agarose slab. Equal portions of a sample were placed at the origin of each running gel and were simultaneously subjected to electrophoresis. Protein within the running gels was detected by staining with Coomassie blue; 0.2% gels were the least concentrated gels that were stained without gel breakage. Using the above technique, the dependence of electrophoretic mobility on agarose concentration has been measured for bacteriophage T7 capsids and a capsid dimer.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 154-159 |
| Number of pages | 6 |
| Journal | Analytical Biochemistry |
| Volume | 101 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1980 |
Fingerprint
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
A technique for electrophoresis in multiple-concentration agarose gels. / Serwer, Philip.
In: Analytical Biochemistry, Vol. 101, No. 1, 01.01.1980, p. 154-159.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A technique for electrophoresis in multiple-concentration agarose gels
AU - Serwer, Philip
PY - 1980/1/1
Y1 - 1980/1/1
N2 - A technique has been developed for embedding several agarose gels (running gels), each of a different agarose concentration, within a single 1.5% agarose slab. Equal portions of a sample were placed at the origin of each running gel and were simultaneously subjected to electrophoresis. Protein within the running gels was detected by staining with Coomassie blue; 0.2% gels were the least concentrated gels that were stained without gel breakage. Using the above technique, the dependence of electrophoretic mobility on agarose concentration has been measured for bacteriophage T7 capsids and a capsid dimer.
AB - A technique has been developed for embedding several agarose gels (running gels), each of a different agarose concentration, within a single 1.5% agarose slab. Equal portions of a sample were placed at the origin of each running gel and were simultaneously subjected to electrophoresis. Protein within the running gels was detected by staining with Coomassie blue; 0.2% gels were the least concentrated gels that were stained without gel breakage. Using the above technique, the dependence of electrophoretic mobility on agarose concentration has been measured for bacteriophage T7 capsids and a capsid dimer.
UR - http://www.scopus.com/inward/record.url?scp=0018877016&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018877016&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(80)90054-8
DO - 10.1016/0003-2697(80)90054-8
M3 - Article
C2 - 7356124
AN - SCOPUS:0018877016
VL - 101
SP - 154
EP - 159
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -