A technique for electrophoresis in multiple-concentration agarose gels

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29 Scopus citations

Abstract

A technique has been developed for embedding several agarose gels (running gels), each of a different agarose concentration, within a single 1.5% agarose slab. Equal portions of a sample were placed at the origin of each running gel and were simultaneously subjected to electrophoresis. Protein within the running gels was detected by staining with Coomassie blue; 0.2% gels were the least concentrated gels that were stained without gel breakage. Using the above technique, the dependence of electrophoretic mobility on agarose concentration has been measured for bacteriophage T7 capsids and a capsid dimer.

Original languageEnglish (US)
Pages (from-to)154-159
Number of pages6
JournalAnalytical Biochemistry
Volume101
Issue number1
DOIs
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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