A technique for electrophoresis in multiple-concentration agarose gels

Philip Serwer

doi:10.1016/0003-2697(80)90054-8

A technique for electrophoresis in multiple-concentration agarose gels

Philip Serwer

Department of Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

A technique has been developed for embedding several agarose gels (running gels), each of a different agarose concentration, within a single 1.5% agarose slab. Equal portions of a sample were placed at the origin of each running gel and were simultaneously subjected to electrophoresis. Protein within the running gels was detected by staining with Coomassie blue; 0.2% gels were the least concentrated gels that were stained without gel breakage. Using the above technique, the dependence of electrophoretic mobility on agarose concentration has been measured for bacteriophage T7 capsids and a capsid dimer.

Original language	English (US)
Pages (from-to)	154-159
Number of pages	6
Journal	Analytical Biochemistry
Volume	101
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(80)90054-8
State	Published - Jan 1 1980

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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10.1016/0003-2697(80)90054-8

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abstract = "A technique has been developed for embedding several agarose gels (running gels), each of a different agarose concentration, within a single 1.5% agarose slab. Equal portions of a sample were placed at the origin of each running gel and were simultaneously subjected to electrophoresis. Protein within the running gels was detected by staining with Coomassie blue; 0.2% gels were the least concentrated gels that were stained without gel breakage. Using the above technique, the dependence of electrophoretic mobility on agarose concentration has been measured for bacteriophage T7 capsids and a capsid dimer.",

author = "Philip Serwer",

note = "Funding Information: For technical assistance during this study I thank Nancy L. Smith and Robert H. Watson. For provision of facilities for electron microscopy I thank Dr. Edward G. Rennels of the Department of Anatomy, The University of Texas Health Science Center at San Antonio. Support was received from Public Health Service Grant GM-2436501 from the National Institute of General Medical Sciences and General Research Support Grant RR 05654 from the General Research Support Branch. Division of Research Resources, National Institutes of Health.",

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AB - A technique has been developed for embedding several agarose gels (running gels), each of a different agarose concentration, within a single 1.5% agarose slab. Equal portions of a sample were placed at the origin of each running gel and were simultaneously subjected to electrophoresis. Protein within the running gels was detected by staining with Coomassie blue; 0.2% gels were the least concentrated gels that were stained without gel breakage. Using the above technique, the dependence of electrophoretic mobility on agarose concentration has been measured for bacteriophage T7 capsids and a capsid dimer.

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A technique for electrophoresis in multiple-concentration agarose gels

Abstract

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