A T-Cell Model for the Biological Role of Selenium-Dependent Glutathione Peroxidase

Alan M. Diamond, Yasushi Kataoka, Judith Murray, Chengying Duan, Thomas M. Folks, Paul A. Sandstrom

    Research output: Contribution to journalArticlepeer-review

    8 Scopus citations

    Abstract

    The cytosolic form of selenium-dependent glutathione peroxidase detoxifies both hydrogen and lipid peroxides and therefore represents a major component of the cellular anti-oxidant defenses. In order to study the biological role of this enzyme, we generated an expression construct in a retroviral vector, which when introduced into immortalized human T-cells, resulted in significant increases in the activity of this important enzyme. This effect is stable over extended maintenance in culture. The anti-oxidant defenses in these same cells are also shown to be attenuated by chemically reducing cellular glutathione levels. Collectively, the ability to both increase and decrease the anti-oxidant defenses in human T cells results in a useful model system for the study of oxidative stress and signaling in this cell type.

    Original languageEnglish (US)
    Pages (from-to)246-252
    Number of pages7
    JournalBiomedical and Environmental Sciences
    Volume10
    Issue number2-3
    StatePublished - Sep 1 1997

    ASJC Scopus subject areas

    • Public Health, Environmental and Occupational Health
    • Health, Toxicology and Mutagenesis

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