A study on skin tumour formation in mice with 50 Hz magnetic field exposure

Agneta Rannug, Tomas Ekström, Kjell Hansson Mild, Bo Holmberg, Irma Gimenez-Conti, Thomas J Slaga

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

In order to test the possibility that magnetic fields (MF) act as a tumour promoter, a long-term skin carcinogenicity study of 50 Hz sinusoidal MF with flux densities of 50 μT and 0.5 mT was performed in female NMRI mice. 7,12-dimethyl-benz[a]anthracene (DMBA) in acetone was applied to the dorsal skin, as an initiator, and exposure to MF was performed for 19 (weekdays) or 21 h/day (weekends and holidays) for 103 weeks starting one week after the initiator treatment. The phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) was used as a positive control for skin tumour promoting activity. MF was also evaluated for complete carcinogenic action in groups of mice that were treated with acetone only. Six animals from each group were taken for skin hyperplasia analysis and were killed after 9, 26 and 52 weeks. The appearance of skin lesions were carefully followed and histopathological diagnosis was made for all neoplasms present at death. The statistical analyses on occurrence of skin tumour bearing animals and cumulated skin tumours, with corrections for survival, did not reveal a difference between the controls and the MF exposed groups. The epithelial thickness of DMBA + MF-treated animals was of the same magnitude as for DMBA-treated animals. Leukaemia was a little more frequent among animals exposed to 0.5 mT MF compared to the control animals. However this difference was not statistically significant.

Original languageEnglish (US)
Pages (from-to)573-578
Number of pages6
JournalCarcinogenesis
Volume14
Issue number4
StatePublished - Apr 1993
Externally publishedYes

Fingerprint

Magnetic Fields
Skin
Mouse
Tumors
Tumor
Magnetic Field
Animals
Magnetic fields
Anthracene
Neoplasms
Acetone
Bearings (structural)
9,10-Dimethyl-1,2-benzanthracene
Holidays
Leukemia
Phorbol Esters
Tetradecanoylphorbol Acetate
Magnetic field
Promoter
Carcinogens

ASJC Scopus subject areas

  • Cancer Research
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience

Cite this

Rannug, A., Ekström, T., Mild, K. H., Holmberg, B., Gimenez-Conti, I., & Slaga, T. J. (1993). A study on skin tumour formation in mice with 50 Hz magnetic field exposure. Carcinogenesis, 14(4), 573-578.

A study on skin tumour formation in mice with 50 Hz magnetic field exposure. / Rannug, Agneta; Ekström, Tomas; Mild, Kjell Hansson; Holmberg, Bo; Gimenez-Conti, Irma; Slaga, Thomas J.

In: Carcinogenesis, Vol. 14, No. 4, 04.1993, p. 573-578.

Research output: Contribution to journalArticle

Rannug, A, Ekström, T, Mild, KH, Holmberg, B, Gimenez-Conti, I & Slaga, TJ 1993, 'A study on skin tumour formation in mice with 50 Hz magnetic field exposure', Carcinogenesis, vol. 14, no. 4, pp. 573-578.
Rannug A, Ekström T, Mild KH, Holmberg B, Gimenez-Conti I, Slaga TJ. A study on skin tumour formation in mice with 50 Hz magnetic field exposure. Carcinogenesis. 1993 Apr;14(4):573-578.
Rannug, Agneta ; Ekström, Tomas ; Mild, Kjell Hansson ; Holmberg, Bo ; Gimenez-Conti, Irma ; Slaga, Thomas J. / A study on skin tumour formation in mice with 50 Hz magnetic field exposure. In: Carcinogenesis. 1993 ; Vol. 14, No. 4. pp. 573-578.
@article{39725722966d40629c34d4d3b5776a7d,
title = "A study on skin tumour formation in mice with 50 Hz magnetic field exposure",
abstract = "In order to test the possibility that magnetic fields (MF) act as a tumour promoter, a long-term skin carcinogenicity study of 50 Hz sinusoidal MF with flux densities of 50 μT and 0.5 mT was performed in female NMRI mice. 7,12-dimethyl-benz[a]anthracene (DMBA) in acetone was applied to the dorsal skin, as an initiator, and exposure to MF was performed for 19 (weekdays) or 21 h/day (weekends and holidays) for 103 weeks starting one week after the initiator treatment. The phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) was used as a positive control for skin tumour promoting activity. MF was also evaluated for complete carcinogenic action in groups of mice that were treated with acetone only. Six animals from each group were taken for skin hyperplasia analysis and were killed after 9, 26 and 52 weeks. The appearance of skin lesions were carefully followed and histopathological diagnosis was made for all neoplasms present at death. The statistical analyses on occurrence of skin tumour bearing animals and cumulated skin tumours, with corrections for survival, did not reveal a difference between the controls and the MF exposed groups. The epithelial thickness of DMBA + MF-treated animals was of the same magnitude as for DMBA-treated animals. Leukaemia was a little more frequent among animals exposed to 0.5 mT MF compared to the control animals. However this difference was not statistically significant.",
author = "Agneta Rannug and Tomas Ekstr{\"o}m and Mild, {Kjell Hansson} and Bo Holmberg and Irma Gimenez-Conti and Slaga, {Thomas J}",
year = "1993",
month = "4",
language = "English (US)",
volume = "14",
pages = "573--578",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - A study on skin tumour formation in mice with 50 Hz magnetic field exposure

AU - Rannug, Agneta

AU - Ekström, Tomas

AU - Mild, Kjell Hansson

AU - Holmberg, Bo

AU - Gimenez-Conti, Irma

AU - Slaga, Thomas J

PY - 1993/4

Y1 - 1993/4

N2 - In order to test the possibility that magnetic fields (MF) act as a tumour promoter, a long-term skin carcinogenicity study of 50 Hz sinusoidal MF with flux densities of 50 μT and 0.5 mT was performed in female NMRI mice. 7,12-dimethyl-benz[a]anthracene (DMBA) in acetone was applied to the dorsal skin, as an initiator, and exposure to MF was performed for 19 (weekdays) or 21 h/day (weekends and holidays) for 103 weeks starting one week after the initiator treatment. The phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) was used as a positive control for skin tumour promoting activity. MF was also evaluated for complete carcinogenic action in groups of mice that were treated with acetone only. Six animals from each group were taken for skin hyperplasia analysis and were killed after 9, 26 and 52 weeks. The appearance of skin lesions were carefully followed and histopathological diagnosis was made for all neoplasms present at death. The statistical analyses on occurrence of skin tumour bearing animals and cumulated skin tumours, with corrections for survival, did not reveal a difference between the controls and the MF exposed groups. The epithelial thickness of DMBA + MF-treated animals was of the same magnitude as for DMBA-treated animals. Leukaemia was a little more frequent among animals exposed to 0.5 mT MF compared to the control animals. However this difference was not statistically significant.

AB - In order to test the possibility that magnetic fields (MF) act as a tumour promoter, a long-term skin carcinogenicity study of 50 Hz sinusoidal MF with flux densities of 50 μT and 0.5 mT was performed in female NMRI mice. 7,12-dimethyl-benz[a]anthracene (DMBA) in acetone was applied to the dorsal skin, as an initiator, and exposure to MF was performed for 19 (weekdays) or 21 h/day (weekends and holidays) for 103 weeks starting one week after the initiator treatment. The phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) was used as a positive control for skin tumour promoting activity. MF was also evaluated for complete carcinogenic action in groups of mice that were treated with acetone only. Six animals from each group were taken for skin hyperplasia analysis and were killed after 9, 26 and 52 weeks. The appearance of skin lesions were carefully followed and histopathological diagnosis was made for all neoplasms present at death. The statistical analyses on occurrence of skin tumour bearing animals and cumulated skin tumours, with corrections for survival, did not reveal a difference between the controls and the MF exposed groups. The epithelial thickness of DMBA + MF-treated animals was of the same magnitude as for DMBA-treated animals. Leukaemia was a little more frequent among animals exposed to 0.5 mT MF compared to the control animals. However this difference was not statistically significant.

UR - http://www.scopus.com/inward/record.url?scp=0027280788&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027280788&partnerID=8YFLogxK

M3 - Article

C2 - 8472317

AN - SCOPUS:0027280788

VL - 14

SP - 573

EP - 578

JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 4

ER -