A spin-label study of membrane proteins and internal microviscosity of erythrocytes in hereditary spherocytosis

G. Bartosz, M. Gaczyńska, E. Grzelińska, L. Judkiewicz

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Electron spin resonance (ESR) spectra of erythrocyte membranes of patients with hereditary spherocytosis (HS) and of healthy controls labeled with a maleimide spin label did not differ significantly both before and after prolonged incubation at 37°C. It suggests that the different behavior of spin-labeled HS erythrocyte membranes upon incubation at a higher temperature reported previously is due indeed to structural abnormalities of HS red cell membranes and not to alterations in their proteolytic activity. Measurements of the rotational correlation time of Tempamine spin probe demonstrated a significant elevation of internal microviscosity of erythrocytes in HS, more pronounced in nonsplenectomized patients.

Original languageEnglish (US)
Pages (from-to)2285-2288
Number of pages4
JournalLife Sciences
Volume41
Issue number20
DOIs
StatePublished - Nov 16 1987
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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