A spatio-temporally compensated acousto-optic scanner for two-photon microscopy providing large field of view

Y. Kremer; J. F. Léger; R. Lapole; N. Honnorat; Y. Candela; S. Dieudonné; L. Bourdieu

doi:10.1364/OE.16.010066

A spatio-temporally compensated acousto-optic scanner for two-photon microscopy providing large field of view

Y. Kremer, J. F. Léger, R. Lapole, N. Honnorat, Y. Candela, S. Dieudonné, L. Bourdieu

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Acousto-optic deflectors (AOD) are promising ultrafast scanners for non-linear microscopy. Their use has been limited until now by their small scanning range and by the spatial and temporal dispersions of the laser beam going through the deflectors. We show that the use of AOD of large aperture (13mm) compared to standard deflectors allows accessing much larger field of view while minimizing spatio-temporal distortions. An acousto-optic modulator (AOM) placed at distance of the AOD is used to compensate spatial and temporal dispersions. Fine tuning of the AOM-AOD setup using a frequency-resolved optical gating (GRENOUILLE) allows elimination of pulse front tilt whereas spatial chirp is minimized thanks to the large aperture AOD.

Original language	English (US)
Pages (from-to)	10066-10076
Number of pages	11
Journal	Optics Express
Volume	16
Issue number	14
DOIs	https://doi.org/10.1364/OE.16.010066
State	Published - Jul 7 2008
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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abstract = "Acousto-optic deflectors (AOD) are promising ultrafast scanners for non-linear microscopy. Their use has been limited until now by their small scanning range and by the spatial and temporal dispersions of the laser beam going through the deflectors. We show that the use of AOD of large aperture (13mm) compared to standard deflectors allows accessing much larger field of view while minimizing spatio-temporal distortions. An acousto-optic modulator (AOM) placed at distance of the AOD is used to compensate spatial and temporal dispersions. Fine tuning of the AOM-AOD setup using a frequency-resolved optical gating (GRENOUILLE) allows elimination of pulse front tilt whereas spatial chirp is minimized thanks to the large aperture AOD.",

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AU - Léger, J. F.

AU - Lapole, R.

AU - Honnorat, N.

AU - Candela, Y.

AU - Dieudonné, S.

AU - Bourdieu, L.

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AB - Acousto-optic deflectors (AOD) are promising ultrafast scanners for non-linear microscopy. Their use has been limited until now by their small scanning range and by the spatial and temporal dispersions of the laser beam going through the deflectors. We show that the use of AOD of large aperture (13mm) compared to standard deflectors allows accessing much larger field of view while minimizing spatio-temporal distortions. An acousto-optic modulator (AOM) placed at distance of the AOD is used to compensate spatial and temporal dispersions. Fine tuning of the AOM-AOD setup using a frequency-resolved optical gating (GRENOUILLE) allows elimination of pulse front tilt whereas spatial chirp is minimized thanks to the large aperture AOD.

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A spatio-temporally compensated acousto-optic scanner for two-photon microscopy providing large field of view

Abstract

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