A simple method to detect Toxoplasma gondii-specific cytotoxic T cells in vivo

Benjamin J. Daniel, Srilakshmi Pandeswara, Michael J. Brumlik, Ai Jie Liu, Suzanne R. Thibodeaux, Sara M. Ludwig, Xiuhua Sun, Tyler J. Curiel

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Cytotoxic T cells (CTLs) are an important component of adaptive immunity. The study of antigen-specific CTLs in vivo is desirable yet difficult. Identification of the class I-restricted peptide used by CTLs for target recognition is often required for detailed studies, but is generally not known for most antigens. Toxoplasma gondii is a medically important, obligate intracellular parasite and is often used as a model for studies of parasite immunology. No class I-restricted peptides for CTLs are known. We show here a new and convenient method to detect T. gondii-specific CTLs in vivo. We engineered T. gondii tachyzoites to express the model antigen ovalbumin, for which many useful reagents and transgenic mice are available. Using ovalbumin-transgenic T. gondii tachyzoites, antigen-specific CTLs were detected in vivo, and at much earlier time points post-infection than previously reported. This new method has several additional advantages over current methods to detect T. gondii-specific CTLs.

Original languageEnglish (US)
Pages (from-to)86-90
Number of pages5
JournalJournal of Immunological Methods
Volume355
Issue number1-2
DOIs
StatePublished - Apr 2010

Keywords

  • CD8
  • Cytotoxic T lymphocytes
  • Toxoplasma gondii

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Daniel, B. J., Pandeswara, S., Brumlik, M. J., Liu, A. J., Thibodeaux, S. R., Ludwig, S. M., Sun, X., & Curiel, T. J. (2010). A simple method to detect Toxoplasma gondii-specific cytotoxic T cells in vivo. Journal of Immunological Methods, 355(1-2), 86-90. https://doi.org/10.1016/j.jim.2010.01.013