A simple method of lung perfusion fixation

J. J. Coalson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A simple perfusion apparatus modified from procedures used and published in renal studies is presented and discussed. The buffer and fixative solutions to be perfused are placed in closed reservoirs under 20 mm of Hg pressure which is provided by a constant inflow of air from an air inflow valve present in any routine laboratory. The pressure is monitored by a sphygmomanometer gauge and adjusted with an air control valve. The perfusate solutions are kept at 37°C. The oncotic-osmotic characteristics of routine buffer and fixative perfusates are presented and discussed. The perfusion technique utilized in a cat model is detailed. The technique does not require sophisticated equipment and yet perfusion conditions can be carefully monitored and controlled.

Original languageEnglish (US)
Pages (from-to)233-238
Number of pages6
JournalAnatomical Record
Volume205
Issue number2
StatePublished - 1983

Fingerprint

Fixatives
valves (equipment)
Perfusion
lungs
Air
air
Lung
Buffers
buffers
Sphygmomanometers
Pressure
gauges
Cats
methodology
kidneys
cats
Kidney
Equipment and Supplies

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Coalson, J. J. (1983). A simple method of lung perfusion fixation. Anatomical Record, 205(2), 233-238.

A simple method of lung perfusion fixation. / Coalson, J. J.

In: Anatomical Record, Vol. 205, No. 2, 1983, p. 233-238.

Research output: Contribution to journalArticle

Coalson, JJ 1983, 'A simple method of lung perfusion fixation', Anatomical Record, vol. 205, no. 2, pp. 233-238.
Coalson, J. J. / A simple method of lung perfusion fixation. In: Anatomical Record. 1983 ; Vol. 205, No. 2. pp. 233-238.
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