A simple method for the quantitation of isozyme patterns

Robert J. Klebe

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Isozyme patterns may be analyzed quantitatively, without the use of a densitometer, by performing serial twofold dilutions of a sample to a visual end point. The specific activity (S) of a given dehydrogenase isozyme can be assessed in the presence of other isozymes catalyzing the same reaction, by (1) determining the isozyme titer (T) (defined as mg protein/ml in the last visible band) and (2) applying the formula S=K/T, where K is 1.6×10-3 units/ml in the last visible band. The units/ml (U) in the starting material can be calculated from the equation U=K (2)n-1, where n is the number of the slot producing the last visible band.

Original languageEnglish (US)
Pages (from-to)805-812
Number of pages8
JournalBiochemical Genetics
Volume13
Issue number11-12
DOIs
StatePublished - Dec 1975
Externally publishedYes

Keywords

  • densitometry
  • isozymes
  • quantitative methods
  • zone electrophoresis

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'A simple method for the quantitation of isozyme patterns'. Together they form a unique fingerprint.

Cite this