A simple device for studying macromolecular crystals under moderate gas pressures (0.1-10 MPa)

Michael H.B. Stowell, S. Michael Soltis, Caroline Kisker, John W. Peters, Hermann Schindelin, D. C. Rees, Duilio Cascio, Lesa Beamer, P. John Hart, Michael C. Wiener, Frank G. Whitby

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

A simple device for studying crystalline samples under moderate gas pressure (0.1-10 MPa) has been developed. The device employs a modified Cajon ultra-torr fitting to ensure a gas-tight seal around an X-ray capillary. The cell accommodates standard X-ray capillaries that require no modification. The device is straightforward to utilize and samples can be mounted with routine techniques and pressurized in a matter of seconds. In a subsequent development, a complete purging and pressurization system has been designed and constructed for use on beamline 7-1 at the Stanford Synchrotron Radiation Laboratory. This paper describes the construction of both the pressure cell and the delivery system and presents results of the use of this cell for the preparation of xenon derivatives to be used in phase determination by the multiple isomorphous replacement method.

Original languageEnglish (US)
Pages (from-to)608-613
Number of pages6
JournalJournal of Applied Crystallography
Volume29
Issue number5
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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