A simple device for studying macromolecular crystals under moderate gas pressures (0.1-10 MPa)

Michael H B Stowell, S. Michael Soltis, Caroline Kisker, John W. Peters, Hermann Schindelin, D. C. Rees, Duilio Cascio, Lesa Beamer, P. John Hart, Michael C. Wiener, Frank G. Whitby

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A simple device for studying crystalline samples under moderate gas pressure (0.1-10 MPa) has been developed. The device employs a modified Cajon ultra-torr fitting to ensure a gas-tight seal around an X-ray capillary. The cell accommodates standard X-ray capillaries that require no modification. The device is straightforward to utilize and samples can be mounted with routine techniques and pressurized in a matter of seconds. In a subsequent development, a complete purging and pressurization system has been designed and constructed for use on beamline 7-1 at the Stanford Synchrotron Radiation Laboratory. This paper describes the construction of both the pressure cell and the delivery system and presents results of the use of this cell for the preparation of xenon derivatives to be used in phase determination by the multiple isomorphous replacement method.

Original languageEnglish (US)
Pages (from-to)608-613
Number of pages6
JournalJournal of Applied Crystallography
Volume29
Issue number5
StatePublished - 1996
Externally publishedYes

Fingerprint

gas pressure
Gases
Pressure
Purging
X rays
Equipment and Supplies
Crystals
Xenon
Pressurization
Synchrotron radiation
cells
X-Rays
purging
crystals
Seals
Synchrotrons
Crystalline materials
Derivatives
xenon
delivery

ASJC Scopus subject areas

  • Structural Biology
  • Condensed Matter Physics

Cite this

Stowell, M. H. B., Soltis, S. M., Kisker, C., Peters, J. W., Schindelin, H., Rees, D. C., ... Whitby, F. G. (1996). A simple device for studying macromolecular crystals under moderate gas pressures (0.1-10 MPa). Journal of Applied Crystallography, 29(5), 608-613.

A simple device for studying macromolecular crystals under moderate gas pressures (0.1-10 MPa). / Stowell, Michael H B; Soltis, S. Michael; Kisker, Caroline; Peters, John W.; Schindelin, Hermann; Rees, D. C.; Cascio, Duilio; Beamer, Lesa; Hart, P. John; Wiener, Michael C.; Whitby, Frank G.

In: Journal of Applied Crystallography, Vol. 29, No. 5, 1996, p. 608-613.

Research output: Contribution to journalArticle

Stowell, MHB, Soltis, SM, Kisker, C, Peters, JW, Schindelin, H, Rees, DC, Cascio, D, Beamer, L, Hart, PJ, Wiener, MC & Whitby, FG 1996, 'A simple device for studying macromolecular crystals under moderate gas pressures (0.1-10 MPa)', Journal of Applied Crystallography, vol. 29, no. 5, pp. 608-613.
Stowell MHB, Soltis SM, Kisker C, Peters JW, Schindelin H, Rees DC et al. A simple device for studying macromolecular crystals under moderate gas pressures (0.1-10 MPa). Journal of Applied Crystallography. 1996;29(5):608-613.
Stowell, Michael H B ; Soltis, S. Michael ; Kisker, Caroline ; Peters, John W. ; Schindelin, Hermann ; Rees, D. C. ; Cascio, Duilio ; Beamer, Lesa ; Hart, P. John ; Wiener, Michael C. ; Whitby, Frank G. / A simple device for studying macromolecular crystals under moderate gas pressures (0.1-10 MPa). In: Journal of Applied Crystallography. 1996 ; Vol. 29, No. 5. pp. 608-613.
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