A silver impregnation technique for the study of steroid receptors in central nervous system tissue prepared for autoradiography

Lyn Richardson, Frank J. Weaker, Peter J. Sheridan

Research output: Contribution to journalArticlepeer-review

Abstract

The commonly used silver stains were found to be unsatisfactory for nervous tissue processed for autoradiography. A silver impregnation procedure for central nervous system tissues prepared for the autoradiographic study of steroid receptors is described. The procedure is a combination of several silver and reticular stains made up in solutions containing dimethylsulfoxide. The technique clearly distinguishes perikarya of neurons, brain nuclei and fiber tracts without substantial loss of silver grains, and thus greatly facilitates the identification of steroid receptor nuclei at all levels of the central nervous system.

Original languageEnglish (US)
Pages (from-to)19-22
Number of pages4
JournalBiotechnic and Histochemistry
Volume57
Issue number1
DOIs
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

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