A sensory neuron-specific, proton-gated ion channel

Chih Cheng Chen, Steven England, Armen N Akopian, John N. Wood

Research output: Contribution to journalArticle

360 Citations (Scopus)

Abstract

Proton-gated channels expressed by sensory neurons are of particular interest because low pH causes pain. Two proton-gated channels, acid-sensing ionic channel (AS/C) and dorsal root ASIC (DRASIC), that are members of the amiloride-sensitive ENaC/Degenerin family are known to be expressed by sensory neurons. Here, we describe the cloning and characterization of an ASIC splice variant, ASIC-β, which contains a unique N-terminal 172 aa, as well as unique 5' and 3' untranslated sequences. ASIC-β, unlike ASIC and DRASIC, is found only in a subset of small and large diameter sensory neurons and is absent from sympathetic neurons or the central nervous system. The patterns of expression of ASIC and ASIC-β transcripts in rat dorsal root ganglion neurons are distinct. When expressed in COS-7 cells, ASIC-β forms a functional channel with electrophysiological properties distinct from ASIC and DRASIC. The pH dependency and sensitivity to amiloride of ASIC-β is similar to that described for ASIC, but unlike ASIC, the channel is not permeable to calcium, nor are ASIC-β-mediated currents inhibited by extracellular calcium. The unique distribution of ASIC-β suggests that it may play a specialized role in sensory neuron function.

Original languageEnglish (US)
Pages (from-to)10240-10245
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number17
DOIs
StatePublished - Aug 18 1998
Externally publishedYes

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Sensory Receptor Cells
Ion Channels
Protons
Spinal Nerve Roots
Amiloride
Degenerin Sodium Channels
Acid Sensing Ion Channels
Calcium
Neurons
COS Cells
Spinal Ganglia
Organism Cloning
Central Nervous System
Pain
Acids

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A sensory neuron-specific, proton-gated ion channel. / Chen, Chih Cheng; England, Steven; Akopian, Armen N; Wood, John N.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 17, 18.08.1998, p. 10240-10245.

Research output: Contribution to journalArticle

Chen, Chih Cheng ; England, Steven ; Akopian, Armen N ; Wood, John N. / A sensory neuron-specific, proton-gated ion channel. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 17. pp. 10240-10245.
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