A sensitive competency assay for concanavalin A binding

Paul M. Horowitz, Philip G. Phillips

Research output: Contribution to journalArticlepeer-review

Abstract

A standardized assay method has been developed for assessing the binding capacity of derivitized concanavalin A (con A). The method takes advantage of the fact that concanavalin A binds to Sephadex G-75 in an α-methyl-d-mannopyranoside-inhibitable manner. Using this binding assay we have found that α-methyl-d-mannopyranoside inhibition of [125I]concanavalin A to Sephadex is greater than 95%. In addition, this sugar is able to remove more than 99% of [125I]concanavalin A previously bound to Sephadex. Competition studies will allow the use of this procedure to characterize any con A sample under the exact conditions used for cell-binding studies.

Original languageEnglish (US)
Pages (from-to)171-174
Number of pages4
JournalAnalytical Biochemistry
Volume74
Issue number1
DOIs
StatePublished - Jul 1976
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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