A sensitive assay for taxol and other microtubule-stabilizing agents

Charles D. Smith, Susan L Mooberry, Xinqun Zhang, Anna Marija Helt

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The ability of taxol to protect microtubules in cultured human ovarian carcinoma cells from drug- and cold-induced depolymerization was characterized as a functional assay for microtubule stabilizing agents. Treatment of the cells with concentrations of vinblastine or colchicine of 50 nM or greater, or incubation at 4°C resulted in complete depolymerization of cytoplasmic microtubules. Pretreatment with taxol for 3 h enabled the cells to maintain substantial numbers of microtubules following the application of vinblastine or colchicine. This protective effect was easily observed at 50 nM taxol, whereas taxol-induced microtubule bundling was observed only at concentrations of 500 nM or greater. Concentrations of taxol as low as 10 nM stabilized microtubules against cold-induced depolymerization. Therefore, protection of microtubules from drug- and cold-induced depolymerization provides a sensitive functional assay for taxol. These systems should be similarly effective in identifying novel compounds which stabilize microtubules.

Original languageEnglish (US)
Pages (from-to)213-219
Number of pages7
JournalCancer Letters
Volume79
Issue number2
DOIs
StatePublished - May 16 1994
Externally publishedYes

Fingerprint

Excipients
Paclitaxel
Microtubules
Vinblastine
Colchicine
Pharmaceutical Preparations
Carcinoma

Keywords

  • Cancer
  • Drug
  • Microtubule
  • Taxol
  • Tubulin

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

A sensitive assay for taxol and other microtubule-stabilizing agents. / Smith, Charles D.; Mooberry, Susan L; Zhang, Xinqun; Helt, Anna Marija.

In: Cancer Letters, Vol. 79, No. 2, 16.05.1994, p. 213-219.

Research output: Contribution to journalArticle

Smith, Charles D. ; Mooberry, Susan L ; Zhang, Xinqun ; Helt, Anna Marija. / A sensitive assay for taxol and other microtubule-stabilizing agents. In: Cancer Letters. 1994 ; Vol. 79, No. 2. pp. 213-219.
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