A screen for mutations that suppress the phenotype of Drosophila armadillo, the β-catenin homolog

Rachel T. Cox, Donald G. McEwen, Denise L. Myster, Robert J. Duronio, Joseph Loureiro, Mark Peifer

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

During development signaling pathways coordinate cell fates and regulate the choice between cell survival or programmed cell death. The well-conserved Wingless/Wnt pathway is required for many developmental decisions in all animals. One transducer of the Wingless/Wnt signal is Armadillo/β-catenin. Drosophila Armadillo not only transduces Wingless signal, but also acts in cell-cell adhesion via its role in the epithelial adherens junction. While many components of both the Wingless/Wnt signaling pathway and adherens junctions are known, both processes are complex, suggesting that unknown components influence signaling and junctions. We carried out a genetic modifier screen to identify some of these components by screening for mutations that can suppress the armadillo mutant phenotype. We identified 12 regions of the genome that have this property. From these regions and from additional candidate genes tested we identified four genes that suppress arm: dTCF, puckered, head involution defective (hid), and Dpresenilin. We further investigated the interaction with hid, a known regulator of programmed cell death. Our data suggest that Wg signaling modulates Hid activity and that Hid regulates programmed cell death in a dose-sensitive fashion.

Original languageEnglish (US)
Pages (from-to)1725-1740
Number of pages16
JournalGenetics
Volume155
Issue number4
StatePublished - Aug 29 2000
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Genetics

Cite this

Cox, R. T., McEwen, D. G., Myster, D. L., Duronio, R. J., Loureiro, J., & Peifer, M. (2000). A screen for mutations that suppress the phenotype of Drosophila armadillo, the β-catenin homolog. Genetics, 155(4), 1725-1740.