A role for calcineurin in the desensitization of the P2X3 receptor

Brian King, Chih Cheng Chen, Armen N Akopian, Geoffrey Burnstock, John N. Wood

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The sensory neurone-specific ATP-gated cation channel P2X3, when expressed in Xenopus oocytes, desensitizes rapidly. Complete removal of extracellular calcium abolishes desensitization. Pretreatment of oocytes with cyclosporin also abolishes P2X3 desensitization. When the calcineurin auto-inhibitory peptide (CaN A457-481) was injected into oocytes, the rate of desensitization of P2X3 decreased on consecutive applications of ATP, suggesting a role for calcineurin in the regulation of desensitization. Truncated P2X3 clones initiating at Met-75 lack the N-terminal intracellular region, but express functional channels in oocytes that do not desensitize. Taken together, these data suggest that P2X3 desensitizes through a calcium-dependent calcineurin-mediated dephosphorylation involving N-terminal residues that are phosphorylated in functional channels.

Original languageEnglish (US)
Pages (from-to)1099-1102
Number of pages4
JournalNeuroReport
Volume8
Issue number5
StatePublished - 1997
Externally publishedYes

Fingerprint

Purinergic P2X3 Receptors
Calcineurin
Oocytes
Adenosine Triphosphate
Calcium
Sensory Receptor Cells
Xenopus
Cyclosporine
Cations
Clone Cells
Peptides

Keywords

  • calcineurin
  • calcium
  • desensitization
  • P2X

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

King, B., Chen, C. C., Akopian, A. N., Burnstock, G., & Wood, J. N. (1997). A role for calcineurin in the desensitization of the P2X3 receptor. NeuroReport, 8(5), 1099-1102.

A role for calcineurin in the desensitization of the P2X3 receptor. / King, Brian; Chen, Chih Cheng; Akopian, Armen N; Burnstock, Geoffrey; Wood, John N.

In: NeuroReport, Vol. 8, No. 5, 1997, p. 1099-1102.

Research output: Contribution to journalArticle

King, B, Chen, CC, Akopian, AN, Burnstock, G & Wood, JN 1997, 'A role for calcineurin in the desensitization of the P2X3 receptor', NeuroReport, vol. 8, no. 5, pp. 1099-1102.
King B, Chen CC, Akopian AN, Burnstock G, Wood JN. A role for calcineurin in the desensitization of the P2X3 receptor. NeuroReport. 1997;8(5):1099-1102.
King, Brian ; Chen, Chih Cheng ; Akopian, Armen N ; Burnstock, Geoffrey ; Wood, John N. / A role for calcineurin in the desensitization of the P2X3 receptor. In: NeuroReport. 1997 ; Vol. 8, No. 5. pp. 1099-1102.
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