Abstract
The sensory neurone-specific ATP-gated cation channel P2X3, when expressed in Xenopus oocytes, desensitizes rapidly. Complete removal of extracellular calcium abolishes desensitization. Pretreatment of oocytes with cyclosporin also abolishes P2X3 desensitization. When the calcineurin auto-inhibitory peptide (CaN A457-481) was injected into oocytes, the rate of desensitization of P2X3 decreased on consecutive applications of ATP, suggesting a role for calcineurin in the regulation of desensitization. Truncated P2X3 clones initiating at Met-75 lack the N-terminal intracellular region, but express functional channels in oocytes that do not desensitize. Taken together, these data suggest that P2X3 desensitizes through a calcium-dependent calcineurin-mediated dephosphorylation involving N-terminal residues that are phosphorylated in functional channels.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1099-1102 |
| Number of pages | 4 |
| Journal | NeuroReport |
| Volume | 8 |
| Issue number | 5 |
| State | Published - 1997 |
| Externally published | Yes |
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Keywords
- calcineurin
- calcium
- desensitization
- P2X
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
A role for calcineurin in the desensitization of the P2X3 receptor. / King, Brian; Chen, Chih Cheng; Akopian, Armen N; Burnstock, Geoffrey; Wood, John N.
In: NeuroReport, Vol. 8, No. 5, 1997, p. 1099-1102.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A role for calcineurin in the desensitization of the P2X3 receptor
AU - King, Brian
AU - Chen, Chih Cheng
AU - Akopian, Armen N
AU - Burnstock, Geoffrey
AU - Wood, John N.
PY - 1997
Y1 - 1997
N2 - The sensory neurone-specific ATP-gated cation channel P2X3, when expressed in Xenopus oocytes, desensitizes rapidly. Complete removal of extracellular calcium abolishes desensitization. Pretreatment of oocytes with cyclosporin also abolishes P2X3 desensitization. When the calcineurin auto-inhibitory peptide (CaN A457-481) was injected into oocytes, the rate of desensitization of P2X3 decreased on consecutive applications of ATP, suggesting a role for calcineurin in the regulation of desensitization. Truncated P2X3 clones initiating at Met-75 lack the N-terminal intracellular region, but express functional channels in oocytes that do not desensitize. Taken together, these data suggest that P2X3 desensitizes through a calcium-dependent calcineurin-mediated dephosphorylation involving N-terminal residues that are phosphorylated in functional channels.
AB - The sensory neurone-specific ATP-gated cation channel P2X3, when expressed in Xenopus oocytes, desensitizes rapidly. Complete removal of extracellular calcium abolishes desensitization. Pretreatment of oocytes with cyclosporin also abolishes P2X3 desensitization. When the calcineurin auto-inhibitory peptide (CaN A457-481) was injected into oocytes, the rate of desensitization of P2X3 decreased on consecutive applications of ATP, suggesting a role for calcineurin in the regulation of desensitization. Truncated P2X3 clones initiating at Met-75 lack the N-terminal intracellular region, but express functional channels in oocytes that do not desensitize. Taken together, these data suggest that P2X3 desensitizes through a calcium-dependent calcineurin-mediated dephosphorylation involving N-terminal residues that are phosphorylated in functional channels.
KW - calcineurin
KW - calcium
KW - desensitization
KW - P2X
UR - http://www.scopus.com/inward/record.url?scp=0030914567&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030914567&partnerID=8YFLogxK
M3 - Article
VL - 8
SP - 1099
EP - 1102
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 5
ER -