A retrotransposon-derived probe for discriminating strains of Cryptococcus neoformans

Suzanne M. Keller, Evelyn A. Hettler, Brian L Wickes

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Hybridization of digested DNA to probes derived from repeated sequences has proven to be an extremely powerful epidemiologic tool for studying the relatedness of fungi. The dispersed nature of these sequences throughout the genome provides the discriminatory power for distinguishing two independent isolates from each other based on banding pattern. The genome of Cryptococcus neoformans contains a number of classes of transposable elements, which are often present in multiple copies. We characterized a probe related to the Ty3/gypsy class of transposable elements called TCN1 and used it to screen multiple isolates from all four serotypes of C. neoformans. DNA with TCN1 homology could be amplified from each isolate of serotypes A and D and all isolates hybridized to a probe derived from TCN1. Isolates from serotype B and C were also tested for the presence of a TCN1 homolog, however, only some of these isolates yielded both a TCN1-specific PCR product or hybridization signal. Comparison of the TCN1 hybridization patterns of serotypes A and D to multiple RAPD patterns of the same isolates suggested that TCN1 was more discriminating and therefore, a useful epidemiological tool.

Original languageEnglish (US)
Pages (from-to)377-387
Number of pages11
JournalMycopathologia
Volume162
Issue number6
DOIs
StatePublished - Dec 2006

Fingerprint

Retroelements
Cryptococcus neoformans
retrotransposons
serotypes
DNA Transposable Elements
transposons
hybridization
Genome
genome
DNA Probes
nucleic acid hybridization
Fungi
Polymerase Chain Reaction
fungi
Serogroup
DNA

Keywords

  • Epidemiology
  • Gattii
  • Genome
  • Ty element

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

Cite this

A retrotransposon-derived probe for discriminating strains of Cryptococcus neoformans. / Keller, Suzanne M.; Hettler, Evelyn A.; Wickes, Brian L.

In: Mycopathologia, Vol. 162, No. 6, 12.2006, p. 377-387.

Research output: Contribution to journalArticle

Keller, Suzanne M. ; Hettler, Evelyn A. ; Wickes, Brian L. / A retrotransposon-derived probe for discriminating strains of Cryptococcus neoformans. In: Mycopathologia. 2006 ; Vol. 162, No. 6. pp. 377-387.
@article{08233b3de0e84e458d93eb0405c97d47,
title = "A retrotransposon-derived probe for discriminating strains of Cryptococcus neoformans",
abstract = "Hybridization of digested DNA to probes derived from repeated sequences has proven to be an extremely powerful epidemiologic tool for studying the relatedness of fungi. The dispersed nature of these sequences throughout the genome provides the discriminatory power for distinguishing two independent isolates from each other based on banding pattern. The genome of Cryptococcus neoformans contains a number of classes of transposable elements, which are often present in multiple copies. We characterized a probe related to the Ty3/gypsy class of transposable elements called TCN1 and used it to screen multiple isolates from all four serotypes of C. neoformans. DNA with TCN1 homology could be amplified from each isolate of serotypes A and D and all isolates hybridized to a probe derived from TCN1. Isolates from serotype B and C were also tested for the presence of a TCN1 homolog, however, only some of these isolates yielded both a TCN1-specific PCR product or hybridization signal. Comparison of the TCN1 hybridization patterns of serotypes A and D to multiple RAPD patterns of the same isolates suggested that TCN1 was more discriminating and therefore, a useful epidemiological tool.",
keywords = "Epidemiology, Gattii, Genome, Ty element",
author = "Keller, {Suzanne M.} and Hettler, {Evelyn A.} and Wickes, {Brian L}",
year = "2006",
month = "12",
doi = "10.1007/s11046-006-0073-2",
language = "English (US)",
volume = "162",
pages = "377--387",
journal = "Mycopathologia",
issn = "0301-486X",
publisher = "Springer Netherlands",
number = "6",

}

TY - JOUR

T1 - A retrotransposon-derived probe for discriminating strains of Cryptococcus neoformans

AU - Keller, Suzanne M.

AU - Hettler, Evelyn A.

AU - Wickes, Brian L

PY - 2006/12

Y1 - 2006/12

N2 - Hybridization of digested DNA to probes derived from repeated sequences has proven to be an extremely powerful epidemiologic tool for studying the relatedness of fungi. The dispersed nature of these sequences throughout the genome provides the discriminatory power for distinguishing two independent isolates from each other based on banding pattern. The genome of Cryptococcus neoformans contains a number of classes of transposable elements, which are often present in multiple copies. We characterized a probe related to the Ty3/gypsy class of transposable elements called TCN1 and used it to screen multiple isolates from all four serotypes of C. neoformans. DNA with TCN1 homology could be amplified from each isolate of serotypes A and D and all isolates hybridized to a probe derived from TCN1. Isolates from serotype B and C were also tested for the presence of a TCN1 homolog, however, only some of these isolates yielded both a TCN1-specific PCR product or hybridization signal. Comparison of the TCN1 hybridization patterns of serotypes A and D to multiple RAPD patterns of the same isolates suggested that TCN1 was more discriminating and therefore, a useful epidemiological tool.

AB - Hybridization of digested DNA to probes derived from repeated sequences has proven to be an extremely powerful epidemiologic tool for studying the relatedness of fungi. The dispersed nature of these sequences throughout the genome provides the discriminatory power for distinguishing two independent isolates from each other based on banding pattern. The genome of Cryptococcus neoformans contains a number of classes of transposable elements, which are often present in multiple copies. We characterized a probe related to the Ty3/gypsy class of transposable elements called TCN1 and used it to screen multiple isolates from all four serotypes of C. neoformans. DNA with TCN1 homology could be amplified from each isolate of serotypes A and D and all isolates hybridized to a probe derived from TCN1. Isolates from serotype B and C were also tested for the presence of a TCN1 homolog, however, only some of these isolates yielded both a TCN1-specific PCR product or hybridization signal. Comparison of the TCN1 hybridization patterns of serotypes A and D to multiple RAPD patterns of the same isolates suggested that TCN1 was more discriminating and therefore, a useful epidemiological tool.

KW - Epidemiology

KW - Gattii

KW - Genome

KW - Ty element

UR - http://www.scopus.com/inward/record.url?scp=33845256446&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845256446&partnerID=8YFLogxK

U2 - 10.1007/s11046-006-0073-2

DO - 10.1007/s11046-006-0073-2

M3 - Article

C2 - 17146581

AN - SCOPUS:33845256446

VL - 162

SP - 377

EP - 387

JO - Mycopathologia

JF - Mycopathologia

SN - 0301-486X

IS - 6

ER -