A retinoic acid-responsive human zinc finger gene, MZF-1, preferentially expressed in myeloid cells

R. Hromas, S. J. Collins, D. Hickstein, W. Raskind, L. L. Deaven, P. O'Hara, F. S. Hagen, K. Kaushansky

Research output: Contribution to journalArticle

159 Scopus citations

Abstract

Zinc finger genes encode metal-binding proteins that can act as transcriptional regulators of other genes. In an effort to identify activators of the genetic cascade in hemopoietic differentiation, we used degenerate synthetic oligonucleotides to the conserved zinc finger histidine-cysteine link to probe a human myeloid λgt11 cDNA library. One of the cDNA clones obtained hybridized preferentially to mRNA from myeloid cells. This cDNA was used to isolate clones encompassing the coding region for this gene. Sequence analysis found 13 zinc finger regions and a glycine-proline-rich region between the fourth and fifth zinc finger domain. The gene was localized to chromosome 19q13.2-4, a chromosome that has a large cluster of zinc finger genes. The gene was preferentially expressed in myeloid leukemia cell lines with the highest mRNA levels noted in HL-60 cells induced to differentiate with retinoic acid. Thus, the new zinc finger gene (designated MZF-1) may be one regulator of transcriptional events during hemopoietic development.

Original languageEnglish (US)
Pages (from-to)14183-14187
Number of pages5
JournalJournal of Biological Chemistry
Volume266
Issue number22
StatePublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Hromas, R., Collins, S. J., Hickstein, D., Raskind, W., Deaven, L. L., O'Hara, P., Hagen, F. S., & Kaushansky, K. (1991). A retinoic acid-responsive human zinc finger gene, MZF-1, preferentially expressed in myeloid cells. Journal of Biological Chemistry, 266(22), 14183-14187.