A reference tissue atlas for the human kidney

Kidney Precision Medicine Project

Research output: Contribution to journalArticlepeer-review

Abstract

Kidney Precision Medicine Project (KPMP) is building a spatially specified human kidney tissue atlas in health and disease with single-cell resolution. Here, we describe the construction of an integrated reference map of cells, pathways, and genes using unaffected regions of nephrectomy tissues and undiseased human biopsies from 56 adult subjects. We use single-cell/nucleus transcriptomics, subsegmental laser microdissection transcriptomics and proteomics, near-single-cell proteomics, 3D and CODEX imaging, and spatial metabolomics to hierarchically identify genes, pathways, and cells. Integrated data from these different technologies coherently identify cell types/subtypes within different nephron segments and the interstitium. These profiles describe cell-level functional organization of the kidney following its physiological functions and link cell subtypes to genes, proteins, metabolites, and pathways. They further show that messenger RNA levels along the nephron are congruent with the subsegmental physiological activity. This reference atlas provides a framework for the classification of kidney disease when multiple molecular mechanisms underlie convergent clinical phenotypes.

Original languageEnglish (US)
Article numbereabn4965
JournalScience Advances
Volume8
Issue number23
DOIs
StatePublished - Jun 2022

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'A reference tissue atlas for the human kidney'. Together they form a unique fingerprint.

Cite this