A rat tracheal model to investigate stent-induced tissue hyperplasia: A pilot study

Ji Hoon Shin, Kyu Bo Sung, Eun Young Kim, Dong Ho Shin, Ho Young Song

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Purpose To evaluate the feasibility of stent placement and the formation of stent-induced tissue hyperplasia and to verify matrix metalloproteinase (MMP) expression in stent-induced tissue hyperplasia in a rat tracheal model. Materials and Methods In a pilot study, four rats were used to verify MMP expression in stent-induced tracheal tissue hyperplasia based on zymography and Western blot analysis. In the main experiment, 12 rats were divided into two groups with different stent-mesh gap sizes (group I, 1.23 × 1.92 mm; group II, 0.80 × 1.80 mm). The stent was 4 mm in diameter and 8 mm long. Follow-up tracheogram was performed before sacrifice 3 weeks after stent placement. The gross and the microscopic findings were evaluated. Results Stent placement was technically successful in all rats. During follow-up, two rats (one from each group) died within 4 days after stent placement, and one stent in group II was placed outside the trachea. MMP-9 expression was significantly higher in tissue hyperplasia than in control normal tissue. The excised specimens showed tissue hyperplasia through the mesh, and all of the stents had become incorporated into the wall of the trachea. The papillary projection thickness, granulation tissue area, percentage of granulation tissue area, and degree of inflammatory cell infiltration were higher in group II than in group I, but there was no statistically significant difference. Conclusions In this study, stent placement was feasible, and the formation of stent-induced tissue hyperplasia was evident in a rat tracheal model. MMP-9 overexpression was evident in the stent-induced tracheal tissue hyperplasia.

Original languageEnglish (US)
Pages (from-to)1878-1883
Number of pages6
JournalJournal of Vascular and Interventional Radiology
Volume21
Issue number12
DOIs
StatePublished - Dec 1 2010
Externally publishedYes

Keywords

  • Abbreviations
  • CI
  • Confidence interval
  • ECM
  • Extracellular matrix
  • Matrix metalloproteinase
  • MMP
  • SD
  • Sprague-Dawley

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

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