A rat burn injury model for studying changes in microvascular permeability

Katie Wiggins-Dohlvik, Binu Tharakan

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

The management of burn patients is an extremely complex and clinically challenging for patient care. Aside from the increasing reports of burn injury and morbidity and mortality directly related to it, the pathobiology of burn trauma is not clearly understood. The rat model of burn trauma described here is currently used in research laboratories to study various aspects of burn injury, including vascular dysfunctions. This model demonstrates the infliction of thermal injury in Sprague-Dawley rats using a well-established boiled water approach. We have utilized intravital microscopy to examine the microvascular hyperpermeability, the excessive leakage of proteins and fluids from the intravascular space to the extravascular space in mesenteric postcapillary venules using this model. An increase in microvascular permeability is a strong indicator of microvascular dysfunctions leading to tissue edema in burn trauma.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages93-100
Number of pages8
DOIs
StatePublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1717
ISSN (Print)1064-3745

Keywords

  • Burn injury
  • Burn trauma
  • Thermal injury
  • Vascular hyperpermeability

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Fingerprint

Dive into the research topics of 'A rat burn injury model for studying changes in microvascular permeability'. Together they form a unique fingerprint.

Cite this