A rapid paper-disc test for penicillinase

K. K. Tu, J. H. Jorgensen, C. W. Stratton

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A practical acidimetric assay for penicillinase production using penicillin and phenol red impregnated in paper discs was developed. Blank discs were first impregnated with buffered penicillin G and, after drying, with 15 phenol red. For use, a disc was added to a bacterial suspension made in saline solution. A yellow color within 1 to 30 min of incubation indicated penicillinase production. These discs were tested against Staphylococcus aureus, Haemophilus influenzae, and Neisseria gonorrhoeae, and the assay was found to be a simple, rapid, and accurate method for detection of penicillinase.

Original languageEnglish (US)
Pages (from-to)557-559
Number of pages3
JournalAmerican Journal of Clinical Pathology
Volume75
Issue number4
StatePublished - 1981
Externally publishedYes

Fingerprint

Penicillinase
Phenolsulfonphthalein
Penicillin G
Neisseria gonorrhoeae
Haemophilus influenzae
Sodium Chloride
Penicillins
Staphylococcus aureus
Suspensions
Color

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Tu, K. K., Jorgensen, J. H., & Stratton, C. W. (1981). A rapid paper-disc test for penicillinase. American Journal of Clinical Pathology, 75(4), 557-559.

A rapid paper-disc test for penicillinase. / Tu, K. K.; Jorgensen, J. H.; Stratton, C. W.

In: American Journal of Clinical Pathology, Vol. 75, No. 4, 1981, p. 557-559.

Research output: Contribution to journalArticle

Tu, KK, Jorgensen, JH & Stratton, CW 1981, 'A rapid paper-disc test for penicillinase', American Journal of Clinical Pathology, vol. 75, no. 4, pp. 557-559.
Tu KK, Jorgensen JH, Stratton CW. A rapid paper-disc test for penicillinase. American Journal of Clinical Pathology. 1981;75(4):557-559.
Tu, K. K. ; Jorgensen, J. H. ; Stratton, C. W. / A rapid paper-disc test for penicillinase. In: American Journal of Clinical Pathology. 1981 ; Vol. 75, No. 4. pp. 557-559.
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