A rapid paper-disc test for penicillinase

K. K. Tu, J. H. Jorgensen, C. W. Stratton

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


A practical acidimetric assay for penicillinase production using penicillin and phenol red impregnated in paper discs was developed. Blank discs were first impregnated with buffered penicillin G and, after drying, with 15 phenol red. For use, a disc was added to a bacterial suspension made in saline solution. A yellow color within 1 to 30 min of incubation indicated penicillinase production. These discs were tested against Staphylococcus aureus, Haemophilus influenzae, and Neisseria gonorrhoeae, and the assay was found to be a simple, rapid, and accurate method for detection of penicillinase.

Original languageEnglish (US)
Pages (from-to)557-559
Number of pages3
JournalAmerican Journal of Clinical Pathology
Issue number4
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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