A rapid assay for von willebrand factor

A. Zaleski, J. D. Olson

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Multiple assays have been described which quantify von Willebrand Factor (vWF) in plasma or other specimens. An assay using a modification of the microtitre method(l) is described. Washed platelets fixed in formalin and frozen in dimethylsulfoxide are substrate for the reaction. Platelets, ristocetin and the specimen to be assayed are combined in the wells of a microtitre plate. The reaction is mixed for defined times and agglutination of platelets read visually. By adjusting the concentration of platelets, concentration of ristoce-tin and time, the sensitivity can be adjusted over a wide range.

Original languageEnglish (US)
Pages (from-to)869-874
Number of pages6
JournalThrombosis Research
Volume46
Issue number6
DOIs
StatePublished - Jun 15 1987

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Keywords

  • ristocetin
  • vWF assay
  • von Willebrand factor

ASJC Scopus subject areas

  • Hematology

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