A rapid assay for von Willebrand factor

A. Zaleski; J. D. Olson

doi:10.1016/0049-3848(88)90383-0

A rapid assay for von Willebrand factor

A. Zaleski, J. D. Olson

Pathology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Multiple assays have been described which quantify von Willebrand Factor (vWF) in plasma or other specimens. An assay using a modification of the microtitre method(1) is described. Washed platelets fixed in formalin and frozen in dimethylsulfoxide are substrate for the reaction. Platelets, ristocetin and the specimen to be assayed are combined in the wells of a microtitre plate. The reaction is mixed for defined times and agglutination of platelets read visually. By adjusting the concentration of platelets, concentration of ristocetin and time, the sensitivity can be adjusted over a wide range.

Original language	English (US)
Pages (from-to)	462-468
Number of pages	7
Journal	Thrombosis Research
Volume	51
Issue number	4
DOIs	https://doi.org/10.1016/0049-3848(88)90383-0
State	Published - Aug 15 1988

ASJC Scopus subject areas

Hematology

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10.1016/0049-3848(88)90383-0

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abstract = "Multiple assays have been described which quantify von Willebrand Factor (vWF) in plasma or other specimens. An assay using a modification of the microtitre method(1) is described. Washed platelets fixed in formalin and frozen in dimethylsulfoxide are substrate for the reaction. Platelets, ristocetin and the specimen to be assayed are combined in the wells of a microtitre plate. The reaction is mixed for defined times and agglutination of platelets read visually. By adjusting the concentration of platelets, concentration of ristocetin and time, the sensitivity can be adjusted over a wide range.",

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AB - Multiple assays have been described which quantify von Willebrand Factor (vWF) in plasma or other specimens. An assay using a modification of the microtitre method(1) is described. Washed platelets fixed in formalin and frozen in dimethylsulfoxide are substrate for the reaction. Platelets, ristocetin and the specimen to be assayed are combined in the wells of a microtitre plate. The reaction is mixed for defined times and agglutination of platelets read visually. By adjusting the concentration of platelets, concentration of ristocetin and time, the sensitivity can be adjusted over a wide range.

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A rapid assay for von Willebrand factor

Abstract

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