A rapid and simple procedure for preparation of γ-32P-labeled nucleoside triphosphates

Roy W. Keenan, Mary K. Zishka, Jonathan S. Nishimura

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

An enzymic procedure has been devised for the synthesis of nucleoside triphosphates labeled with 32P in the γ-position. Conditions have been determined that lead to high yields of the radioactive products which have been shown to be labeled only in the γ-phosphate. This method is especially convenient and rapid because all of the enzymes and substrates required are commercially available.

Original languageEnglish (US)
Pages (from-to)601-608
Number of pages8
JournalAnalytical Biochemistry
Volume47
Issue number2
DOIs
StatePublished - Jun 1972
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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