A rapid and simple procedure for preparation of γ-32P-labeled nucleoside triphosphates

Roy W. Keenan; Mary K. Zishka; Jonathan S. Nishimura

doi:10.1016/0003-2697(72)90154-6

A rapid and simple procedure for preparation of γ-³²P-labeled nucleoside triphosphates

Roy W. Keenan, Mary K. Zishka, Jonathan S. Nishimura

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

An enzymic procedure has been devised for the synthesis of nucleoside triphosphates labeled with ³²P in the γ-position. Conditions have been determined that lead to high yields of the radioactive products which have been shown to be labeled only in the γ-phosphate. This method is especially convenient and rapid because all of the enzymes and substrates required are commercially available.

Original language	English (US)
Pages (from-to)	601-608
Number of pages	8
Journal	Analytical Biochemistry
Volume	47
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(72)90154-6
State	Published - Jun 1972
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(72)90154-6

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title = "A rapid and simple procedure for preparation of γ-32P-labeled nucleoside triphosphates",

abstract = "An enzymic procedure has been devised for the synthesis of nucleoside triphosphates labeled with 32P in the γ-position. Conditions have been determined that lead to high yields of the radioactive products which have been shown to be labeled only in the γ-phosphate. This method is especially convenient and rapid because all of the enzymes and substrates required are commercially available.",

author = "Keenan, {Roy W.} and Zishka, {Mary K.} and Nishimura, {Jonathan S.}",

note = "Funding Information: {\textquoteleft}This work was supported by grant GM 13742 from the U. S. Public Service and grant GM 15586 from the National Science Foundation. {\textquoteleft}Present address: The Medical College of Pennsylvania, 3300 Henry Philadelphia, Pennsylvania, 19129.",

year = "1972",

month = jun,

doi = "10.1016/0003-2697(72)90154-6",

language = "English (US)",

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journal = "Analytical Biochemistry",

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T1 - A rapid and simple procedure for preparation of γ-32P-labeled nucleoside triphosphates

AU - Keenan, Roy W.

AU - Zishka, Mary K.

AU - Nishimura, Jonathan S.

N1 - Funding Information: ‘This work was supported by grant GM 13742 from the U. S. Public Service and grant GM 15586 from the National Science Foundation. ‘Present address: The Medical College of Pennsylvania, 3300 Henry Philadelphia, Pennsylvania, 19129.

PY - 1972/6

Y1 - 1972/6

N2 - An enzymic procedure has been devised for the synthesis of nucleoside triphosphates labeled with 32P in the γ-position. Conditions have been determined that lead to high yields of the radioactive products which have been shown to be labeled only in the γ-phosphate. This method is especially convenient and rapid because all of the enzymes and substrates required are commercially available.

AB - An enzymic procedure has been devised for the synthesis of nucleoside triphosphates labeled with 32P in the γ-position. Conditions have been determined that lead to high yields of the radioactive products which have been shown to be labeled only in the γ-phosphate. This method is especially convenient and rapid because all of the enzymes and substrates required are commercially available.

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

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ER -

A rapid and simple procedure for preparation of γ-³²P-labeled nucleoside triphosphates

Abstract

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