A rapid and reliable PCR-based assay for gene transmission and sex determination in newborn transgenic mice

Yan Lavrovsky, Chung Seog Song, Bandana Chatterjee, Arun K. Roy

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

This article describes a reliable and rapid method for simultaneous detection of a transgene and sex determination in the newborn mouse pups by PCR using three sets of primers in a single reaction. One set of sense/antisense primers is used to amplify the experimental transgene (androgen receptor gene in this case), the second set for the mouse Y-chromosome-specific SRY gene, and the third set for the β subunit of the thyroid stimulating hormone (TSHβ), an internal control. This procedure allowed us to promptly analyze pups born from transgenic founders carrying the androgen receptor transgene and, at the same time, establish the sex of the animals. The method is simple, rapid and highly reproducible.

Original languageEnglish (US)
Pages (from-to)319-320
Number of pages2
JournalTransgenic Research
Volume7
Issue number4
DOIs
StatePublished - Jul 1 1998

Keywords

  • Androgen receptor
  • PCR
  • SRY
  • Sex
  • Transgenic mice

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Agronomy and Crop Science
  • Genetics

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