A radiation hybrid map of the BRCA1 region

P. O'Connell, H. Albertsen, N. Matsunami, T. Taylor, J. E. Hundley, T. L. Johnson-Pais, B. Reus, E. Lawrence, L. Ballard, R. White, R. J. Leach

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

A locus on chromosome 17q, designated 'BRCA1,' has been identified as a predisposition gene for breast cancer. A panel of chromosome 17-specific radiation-reduced somatic cell hybrid clones has been assembled for high- resolution mapping of chromosome 17. A series of 35 markers, known to span the BRCA1 locus, were tested against this hybrid panel by PCR assays. Statistical analysis of these data yields a BRCA1 radiation hybrid map at a density sufficient to initiate YAC cloning and pulsed-field gel electrophoretic mapping of the candidate region. In addition, many of the markers reveal genetic polymorphisms and may be tested in breast cancer families and in loss-of-heterozygosity studies of sporadic breast cancers to better define the BRCA1 gene candidate region.

Original languageEnglish (US)
Pages (from-to)526-534
Number of pages9
JournalAmerican Journal of Human Genetics
Volume54
Issue number3
StatePublished - 1994

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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