A radiation hybrid map of 40 loci for the distal long arm of human chromosome 8

Tracey B. Lewis; Lesa Nelson; Ken Ward; Robin J. Leach

doi:10.1101/gr.5.4.334

A radiation hybrid map of 40 loci for the distal long arm of human chromosome 8

Tracey B. Lewis, Lesa Nelson, Ken Ward, Robin J. Leach

Department of Cell Systems & Anatomy

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We generated a panel of 97 radiation hybrids from the cell line GM10156B, which contains only human chromosome 8 in a Chinese hamster ovary cell line background. Statistical analysis of the cosegregation of markers in the 97 radiation hybrids was used to construct a physical map delineating the order and intermarker distance of 40 8q24 loci. Twenty one loci were ordered with maximum likelihood ratios greater than 1000:1. A high level of consistancy was seen between our RH map and the published genetic map, suggesting that our panel will be a valuable resource for the rapid mapping of markers derived from human chromosome 8.

Original language	English (US)
Pages (from-to)	334-341
Number of pages	8
Journal	Genome Research
Volume	5
Issue number	4
DOIs	https://doi.org/10.1101/gr.5.4.334
State	Published - Nov 1995

ASJC Scopus subject areas

Genetics(clinical)
Genetics

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title = "A radiation hybrid map of 40 loci for the distal long arm of human chromosome 8",

abstract = "We generated a panel of 97 radiation hybrids from the cell line GM10156B, which contains only human chromosome 8 in a Chinese hamster ovary cell line background. Statistical analysis of the cosegregation of markers in the 97 radiation hybrids was used to construct a physical map delineating the order and intermarker distance of 40 8q24 loci. Twenty one loci were ordered with maximum likelihood ratios greater than 1000:1. A high level of consistancy was seen between our RH map and the published genetic map, suggesting that our panel will be a valuable resource for the rapid mapping of markers derived from human chromosome 8.",

author = "Lewis, {Tracey B.} and Lesa Nelson and Ken Ward and Leach, {Robin J.}",

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AU - Nelson, Lesa

AU - Ward, Ken

AU - Leach, Robin J.

PY - 1995/11

Y1 - 1995/11

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AB - We generated a panel of 97 radiation hybrids from the cell line GM10156B, which contains only human chromosome 8 in a Chinese hamster ovary cell line background. Statistical analysis of the cosegregation of markers in the 97 radiation hybrids was used to construct a physical map delineating the order and intermarker distance of 40 8q24 loci. Twenty one loci were ordered with maximum likelihood ratios greater than 1000:1. A high level of consistancy was seen between our RH map and the published genetic map, suggesting that our panel will be a valuable resource for the rapid mapping of markers derived from human chromosome 8.

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A radiation hybrid map of 40 loci for the distal long arm of human chromosome 8

Abstract

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