TY - JOUR
T1 - A quantitative test for copper using bicinchoninic acid
AU - Brenner, Andrew J.
AU - Harris, Edward D.
PY - 1995/3
Y1 - 1995/3
N2 - We describe a direct colorimetric assay for copper in serum and biological samples using 2,2′-bicinchoninic acid (BCA), a common reagent in most laboratories. BCA offers the advantage of being highly sensitive and specific for Cu(I) which rapidly forms an intense purple complex in the presence of BCA. The complex has peak absorbances at 562 and 354.5 nm with molar absorptivities of 7.7 × 103 liter mol-1 cm-1 and 4.6 × 104 liter mol-1 cm-1, respectively. Interference by other metal ions, pH, and detergents is minimal and the results correlate strongly with atomic absorption spectrophotometry.
AB - We describe a direct colorimetric assay for copper in serum and biological samples using 2,2′-bicinchoninic acid (BCA), a common reagent in most laboratories. BCA offers the advantage of being highly sensitive and specific for Cu(I) which rapidly forms an intense purple complex in the presence of BCA. The complex has peak absorbances at 562 and 354.5 nm with molar absorptivities of 7.7 × 103 liter mol-1 cm-1 and 4.6 × 104 liter mol-1 cm-1, respectively. Interference by other metal ions, pH, and detergents is minimal and the results correlate strongly with atomic absorption spectrophotometry.
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U2 - 10.1006/abio.1995.1194
DO - 10.1006/abio.1995.1194
M3 - Article
C2 - 7785783
AN - SCOPUS:0028945240
SN - 0003-2697
VL - 226
SP - 80
EP - 84
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -