A primary study on genes with selected mutations by in vitro passage of Chlamydia muridarum strains

Zhou Zhou, Na Liu, Yingzi Wang, Arthur Wirekoh Emmanuel, Xiaoxing You, Jiulin Liu, Zhongyu Li, Yimou Wu, Guangming Zhong

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: This study is to investigate the functions of newly discovered genes in Chlamydia muridarum (C. muridarum) strains with single gene differences. METHODS: Using whole genome sequencing and plaque formation assays, C. muridarum parental and passaging strains were established, and the isogenic clones expressing certain genotypes were isolated. Strains with single gene differences were obtained. Based on prediction, the valuable strains with single gene differences of tc0412, tc0668 or tc0237 were subjected to the in vitro and in vivo experiments for biological characterization and virulence analysis. RESULTS: Insertional -472840T mutation of the tc0412 gene (T28T/B3 type) matching with the nonmutant tc0668 gene and tc0237 gene with point mutations G797659T (Q117E) might slow the growth of Chlamydia due to the lack of a plasmid. The nonmutant tc0668 in the strain might induce a high incidence of hydrosalpinx in mice, while tc0668 with a G797659T point mutation was significantly attenuated. Compared with the nonmutant tc0237, the strains containing mutant tc0237 were characterized by reduced centrifugation dependence during infection. CONCLUSION: The identification and characterization of these genes might contribute to the comprehensive understanding of the pathogenic mechanism of Chlamydia.

Original languageEnglish (US)
JournalPathogens and disease
Volume77
Issue number3
DOIs
StatePublished - Apr 1 2019

Fingerprint

Chlamydia muridarum
Mutation
Genes
Chlamydia
Point Mutation
In Vitro Techniques
Centrifugation
Virulence
Plasmids
Clone Cells
Genotype
Genome

Keywords

  • Chlamydia muridarum (C. muridarum)
  • biological characteristics
  • pathogenic mechanism
  • urogenital infection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

Cite this

A primary study on genes with selected mutations by in vitro passage of Chlamydia muridarum strains. / Zhou, Zhou; Liu, Na; Wang, Yingzi; Emmanuel, Arthur Wirekoh; You, Xiaoxing; Liu, Jiulin; Li, Zhongyu; Wu, Yimou; Zhong, Guangming.

In: Pathogens and disease, Vol. 77, No. 3, 01.04.2019.

Research output: Contribution to journalArticle

Zhou, Zhou ; Liu, Na ; Wang, Yingzi ; Emmanuel, Arthur Wirekoh ; You, Xiaoxing ; Liu, Jiulin ; Li, Zhongyu ; Wu, Yimou ; Zhong, Guangming. / A primary study on genes with selected mutations by in vitro passage of Chlamydia muridarum strains. In: Pathogens and disease. 2019 ; Vol. 77, No. 3.
@article{7fc647a63dac435db76d6c0b2cb769f1,
title = "A primary study on genes with selected mutations by in vitro passage of Chlamydia muridarum strains",
abstract = "OBJECTIVE: This study is to investigate the functions of newly discovered genes in Chlamydia muridarum (C. muridarum) strains with single gene differences. METHODS: Using whole genome sequencing and plaque formation assays, C. muridarum parental and passaging strains were established, and the isogenic clones expressing certain genotypes were isolated. Strains with single gene differences were obtained. Based on prediction, the valuable strains with single gene differences of tc0412, tc0668 or tc0237 were subjected to the in vitro and in vivo experiments for biological characterization and virulence analysis. RESULTS: Insertional -472840T mutation of the tc0412 gene (T28T/B3 type) matching with the nonmutant tc0668 gene and tc0237 gene with point mutations G797659T (Q117E) might slow the growth of Chlamydia due to the lack of a plasmid. The nonmutant tc0668 in the strain might induce a high incidence of hydrosalpinx in mice, while tc0668 with a G797659T point mutation was significantly attenuated. Compared with the nonmutant tc0237, the strains containing mutant tc0237 were characterized by reduced centrifugation dependence during infection. CONCLUSION: The identification and characterization of these genes might contribute to the comprehensive understanding of the pathogenic mechanism of Chlamydia.",
keywords = "Chlamydia muridarum (C. muridarum), biological characteristics, pathogenic mechanism, urogenital infection",
author = "Zhou Zhou and Na Liu and Yingzi Wang and Emmanuel, {Arthur Wirekoh} and Xiaoxing You and Jiulin Liu and Zhongyu Li and Yimou Wu and Guangming Zhong",
year = "2019",
month = "4",
day = "1",
doi = "10.1093/femspd/ftz017",
language = "English (US)",
volume = "77",
journal = "Pathogens and Disease",
issn = "2049-632X",
publisher = "John Wiley & Sons Inc.",
number = "3",

}

TY - JOUR

T1 - A primary study on genes with selected mutations by in vitro passage of Chlamydia muridarum strains

AU - Zhou, Zhou

AU - Liu, Na

AU - Wang, Yingzi

AU - Emmanuel, Arthur Wirekoh

AU - You, Xiaoxing

AU - Liu, Jiulin

AU - Li, Zhongyu

AU - Wu, Yimou

AU - Zhong, Guangming

PY - 2019/4/1

Y1 - 2019/4/1

N2 - OBJECTIVE: This study is to investigate the functions of newly discovered genes in Chlamydia muridarum (C. muridarum) strains with single gene differences. METHODS: Using whole genome sequencing and plaque formation assays, C. muridarum parental and passaging strains were established, and the isogenic clones expressing certain genotypes were isolated. Strains with single gene differences were obtained. Based on prediction, the valuable strains with single gene differences of tc0412, tc0668 or tc0237 were subjected to the in vitro and in vivo experiments for biological characterization and virulence analysis. RESULTS: Insertional -472840T mutation of the tc0412 gene (T28T/B3 type) matching with the nonmutant tc0668 gene and tc0237 gene with point mutations G797659T (Q117E) might slow the growth of Chlamydia due to the lack of a plasmid. The nonmutant tc0668 in the strain might induce a high incidence of hydrosalpinx in mice, while tc0668 with a G797659T point mutation was significantly attenuated. Compared with the nonmutant tc0237, the strains containing mutant tc0237 were characterized by reduced centrifugation dependence during infection. CONCLUSION: The identification and characterization of these genes might contribute to the comprehensive understanding of the pathogenic mechanism of Chlamydia.

AB - OBJECTIVE: This study is to investigate the functions of newly discovered genes in Chlamydia muridarum (C. muridarum) strains with single gene differences. METHODS: Using whole genome sequencing and plaque formation assays, C. muridarum parental and passaging strains were established, and the isogenic clones expressing certain genotypes were isolated. Strains with single gene differences were obtained. Based on prediction, the valuable strains with single gene differences of tc0412, tc0668 or tc0237 were subjected to the in vitro and in vivo experiments for biological characterization and virulence analysis. RESULTS: Insertional -472840T mutation of the tc0412 gene (T28T/B3 type) matching with the nonmutant tc0668 gene and tc0237 gene with point mutations G797659T (Q117E) might slow the growth of Chlamydia due to the lack of a plasmid. The nonmutant tc0668 in the strain might induce a high incidence of hydrosalpinx in mice, while tc0668 with a G797659T point mutation was significantly attenuated. Compared with the nonmutant tc0237, the strains containing mutant tc0237 were characterized by reduced centrifugation dependence during infection. CONCLUSION: The identification and characterization of these genes might contribute to the comprehensive understanding of the pathogenic mechanism of Chlamydia.

KW - Chlamydia muridarum (C. muridarum)

KW - biological characteristics

KW - pathogenic mechanism

KW - urogenital infection

UR - http://www.scopus.com/inward/record.url?scp=85068487564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068487564&partnerID=8YFLogxK

U2 - 10.1093/femspd/ftz017

DO - 10.1093/femspd/ftz017

M3 - Article

C2 - 31197357

AN - SCOPUS:85068487564

VL - 77

JO - Pathogens and Disease

JF - Pathogens and Disease

SN - 2049-632X

IS - 3

ER -