A potential role for colony-stimulating factor 1 in the genesis of the early endometriotic lesion

Jani R. Jensen, Craig A. Witz, Robert S. Schenken, Rajeshwar R. Tekmal

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Objective: To investigate the role(s) of colony-stimulating factor 1 (CSF-1) on the development of early endometriosis in a murine model by comparing rate of lesion formation in mice [1] homozygous for a CSF-1 mutation versus syngeneic controls and [2] after treatment with imatinib, a commercially available tyrosine kinase inhibitor that alters interaction(s) between CSF-1 and its receptor, c-fms. Design: Prospective, placebo-controlled animal study. Setting: Academic medical center. Animals: Six- to 8-week old female FVB, wild-type C57BL/6, and CSF-1 op/op mice. Intervention(s): Endometrial tissue from donor mice was used to induce endometriosis in murine recipients. In some experiments, mice homozygous for a CSF-1 mutation (CSF-1 op/op) were donors or recipients. In other experiments, donor and/or recipient mice received imatinib. Main Outcome Measure(s): Histologic confirmation of endometriosis, rate of lesion formation. Result(s): By 40 hours, recipient mice developed a mean of 7.2 ± 0.9 endometriotic lesions that had invaded host surfaces, and mesothelial cells had proliferated over the entire surface of the implants. The CSF-1 op/op mice developed significantly fewer (mean 0.9 ± 0.3) endometriotic lesions versus syngeneic controls. Imatinib treatment resulted in significantly fewer lesions when compared with sham-treated controls. Conclusion(s): Colony-stimulating factor 1 has a role in establishing early endometriotic lesions. Agents targeting CSF-1 or its actions have therapeutic potential for treating endometriosis.

Original languageEnglish (US)
Pages (from-to)251-256
Number of pages6
JournalFertility and Sterility
Volume93
Issue number1
DOIs
StatePublished - Jan 1 2010

Keywords

  • CSF-1
  • Endometriosis
  • animal model
  • colony-stimulating factor 1
  • imatinib

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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