A Pit-1 binding site 3′ to the transcription start site inhibits transcription elongation in vitro

Kelly P. Smith, Z. Dave Sharp

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The transcription factor Pit-1 can autoregulate its own expression by interacting with binding sites associated with the Pit-1 gene. One of these binding sites is located within the transcribed region of the gene and appears to be responsible for negatively regulating Pit-1 transcription. Using unique constructions in an in vitro transcription system, we show here that binding of Pit-1 to its recognition element can impede the progress of transcription elongation through the occupied site. We propose that this is a viable mechanism for Pit-1 autoregulation.

Original languageEnglish (US)
Pages (from-to)790-796
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume177
Issue number2
DOIs
StatePublished - Jun 14 1991

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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