A phylogenetic analysis of filarial nematodes: Comparison with the phylogeny of Wolbachia endosymbionts

M. Casiraghi, T. J.C. Anderson, C. Bandi, C. Bazzocchi, C. Genchi

    Research output: Contribution to journalArticle

    259 Scopus citations

    Abstract

    Infection with the endosymbiotic bacteria Wolbachia is widespread in filarial nematodes. Previous studies have suggested concordance between the phylogeny of Wolbachia with that of their nematode hosts. However, there is only one published molecular phylogenetic study of filarial species, based on the 5S rRNA gene spacer. The phylogeny proposed by this study is partially incongruent with previous classifications of filarial nematodes, based on morphological characters. Furthermore, both traditional classifications and molecular phylogenies are, in part, inconsistent with the phylogeny of Wolbachia. Here we report mitochondrial cytochrome oxidase I (COI) gene sequences for 11 species of filaria and for another spirurid nematode which was included as an outgroup. In addition, 16S rRNA, wsp and ftsZ gene sequences were generated for the Wolbachia of several filarial species, in order to complete the available data sets and further resolve the phylogeny of Wolbachia in nematodes. We used these data to evaluate whether nematode and Wolbachia phylogenies are concordant. Some of the possible phylogenetic reconstructions based on COI gene were congruent with the phylogeny of Wolbachia and supported the grouping of the rodent filaria Litomosoides sigmodontis with the lymphatic filariae (i.e. Brugia spp. and Wuchereria spp.) and the sister group relationship of Dirofilaria spp. and Onchocerca spp. However, the placement of the Wolbachia-free filaria Acanthocheilonema viteae is ambiguous and dependent on the phylogenetic methods used.

    Original languageEnglish (US)
    Pages (from-to)93-103
    Number of pages11
    JournalParasitology
    Volume122
    Issue number1
    DOIs
    StatePublished - Feb 22 2001

    Keywords

    • 16S rDNA
    • COI gene
    • Filarial nematodes
    • Phylogeny
    • Wolbachia
    • ftsZ
    • wsp

    ASJC Scopus subject areas

    • Parasitology
    • Animal Science and Zoology
    • Infectious Diseases

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