A phylogenetic analysis of filarial nematodes: Comparison with the phylogeny of Wolbachia endosymbionts

M. Casiraghi, T. J C Anderson, C. Bandi, C. Bazzocchi, C. Genchi

Research output: Contribution to journalArticle

246 Citations (Scopus)

Abstract

Infection with the endosymbiotic bacteria Wolbachia is widespread in filarial nematodes. Previous studies have suggested concordance between the phylogeny of Wolbachia with that of their nematode hosts. However, there is only one published molecular phylogenetic study of filarial species, based on the 5S rRNA gene spacer. The phylogeny proposed by this study is partially incongruent with previous classifications of filarial nematodes, based on morphological characters. Furthermore, both traditional classifications and molecular phylogenies are, in part, inconsistent with the phylogeny of Wolbachia. Here we report mitochondrial cytochrome oxidase I (COI) gene sequences for 11 species of filaria and for another spirurid nematode which was included as an outgroup. In addition, 16S rRNA, wsp and ftsZ gene sequences were generated for the Wolbachia of several filarial species, in order to complete the available data sets and further resolve the phylogeny of Wolbachia in nematodes. We used these data to evaluate whether nematode and Wolbachia phylogenies are concordant. Some of the possible phylogenetic reconstructions based on COI gene were congruent with the phylogeny of Wolbachia and supported the grouping of the rodent filaria Litomosoides sigmodontis with the lymphatic filariae (i.e. Brugia spp. and Wuchereria spp.) and the sister group relationship of Dirofilaria spp. and Onchocerca spp. However, the placement of the Wolbachia-free filaria Acanthocheilonema viteae is ambiguous and dependent on the phylogenetic methods used.

Original languageEnglish (US)
Pages (from-to)93-103
Number of pages11
JournalParasitology
Volume122
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Wolbachia
endosymbionts
Phylogeny
Nematoda
phylogeny
Electron Transport Complex IV
Wuchereria
Acanthocheilonema
Brugia
Dirofilaria
Onchocerca
Filarioidea
cytochrome-c oxidase
Genes
Acanthocheilonema viteae
ribosomal RNA
taxonomy
nucleotide sequences
rRNA Genes
Rodentia

Keywords

  • 16S rDNA
  • COI gene
  • Filarial nematodes
  • ftsZ
  • Phylogeny
  • Wolbachia
  • wsp

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

A phylogenetic analysis of filarial nematodes : Comparison with the phylogeny of Wolbachia endosymbionts. / Casiraghi, M.; Anderson, T. J C; Bandi, C.; Bazzocchi, C.; Genchi, C.

In: Parasitology, Vol. 122, No. 1, 2001, p. 93-103.

Research output: Contribution to journalArticle

Casiraghi, M, Anderson, TJC, Bandi, C, Bazzocchi, C & Genchi, C 2001, 'A phylogenetic analysis of filarial nematodes: Comparison with the phylogeny of Wolbachia endosymbionts', Parasitology, vol. 122, no. 1, pp. 93-103. https://doi.org/10.1017/S0031182000007149
Casiraghi, M. ; Anderson, T. J C ; Bandi, C. ; Bazzocchi, C. ; Genchi, C. / A phylogenetic analysis of filarial nematodes : Comparison with the phylogeny of Wolbachia endosymbionts. In: Parasitology. 2001 ; Vol. 122, No. 1. pp. 93-103.
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