A pH-sensitive eosin-block copolymer delivers proteins intracellularly

Sören Reinhard; Hesong Han; Jan Tuma; Joachim Justad Røise; I. Che Li; Jie Li; Hye Young Lee; Niren Murthy

doi:10.1039/d0cc05165a

A pH-sensitive eosin-block copolymer delivers proteins intracellularly

Sören Reinhard, Hesong Han, Jan Tuma, Joachim Justad Røise, I. Che Li, Jie Li, Hye Young Lee, Niren Murthy

Department of Cellular & Integrative Physiology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

There is great interest in developing strategies to deliver proteins into the cytoplasm of cells. We report here a PEG-poly-eosin block copolymer (PEG-pEosin) that can encapsulate proteins and release them in active form under mildly acidic conditions. A PEG-pEosin formulation composed of Cre and the endosomolytic protein LLO efficiently performed gene editing in cells and in the brains of mice after an intracranial injection.

Original language	English (US)
Pages (from-to)	14207-14210
Number of pages	4
Journal	Chemical Communications
Volume	56
Issue number	91
DOIs	https://doi.org/10.1039/d0cc05165a
State	Published - Nov 25 2020

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Chemistry(all)
Catalysis

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abstract = "There is great interest in developing strategies to deliver proteins into the cytoplasm of cells. We report here a PEG-poly-eosin block copolymer (PEG-pEosin) that can encapsulate proteins and release them in active form under mildly acidic conditions. A PEG-pEosin formulation composed of Cre and the endosomolytic protein LLO efficiently performed gene editing in cells and in the brains of mice after an intracranial injection.",

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AU - Reinhard, Sören

AU - Han, Hesong

AU - Tuma, Jan

AU - Røise, Joachim Justad

AU - Li, I. Che

AU - Li, Jie

AU - Lee, Hye Young

AU - Murthy, Niren

PY - 2020/11/25

Y1 - 2020/11/25

N2 - There is great interest in developing strategies to deliver proteins into the cytoplasm of cells. We report here a PEG-poly-eosin block copolymer (PEG-pEosin) that can encapsulate proteins and release them in active form under mildly acidic conditions. A PEG-pEosin formulation composed of Cre and the endosomolytic protein LLO efficiently performed gene editing in cells and in the brains of mice after an intracranial injection.

AB - There is great interest in developing strategies to deliver proteins into the cytoplasm of cells. We report here a PEG-poly-eosin block copolymer (PEG-pEosin) that can encapsulate proteins and release them in active form under mildly acidic conditions. A PEG-pEosin formulation composed of Cre and the endosomolytic protein LLO efficiently performed gene editing in cells and in the brains of mice after an intracranial injection.

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SP - 14207

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JO - Chemical Communications

JF - Chemical Communications

IS - 91

ER -

A pH-sensitive eosin-block copolymer delivers proteins intracellularly

Abstract

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