A peripheral nervous system actin-binding protein regulates neurite outgrowth

Samantha J. Ravenall, Isabella Gavazzi, John N. Wood, Armen N. Akopian

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Difference cloning has identified a Villin-like mRNA transcript expressed selectively in peripheral sensory and sympathetic neurons. Pervin, the encoded 820-amino acid protein, has 60% identity with Villin and is the rat homologue of Advillin. Coimmunoprecipitation studies demonstrate that Pervin and actin interact in vivo. Transfection of COS-7 epithelial cell lines demonstrates colocalization of epitope-tagged Pervin with green fluorescent protein-actin and results in an increase in process formation. This effect is abolished when the putative actin-bundling headpiece of Pervin is deleted. Biolistic transfection of primary cultures of rat dorsal root ganglion sensory neurons also results in increased neurite outgrowth with FLAG-tagged Pervin. Deletion of the actin-bundling headpiece inhibits normal neurite growth. These data suggest that Pervin may play a significant role in regulating process outgrowth in peripheral neurons through a mechanism that involves the activity of an actin-bundling domain.

Original languageEnglish (US)
Pages (from-to)281-290
Number of pages10
JournalEuropean Journal of Neuroscience
Issue number2
StatePublished - 2002
Externally publishedYes


  • Actin-binding protein
  • Neurite outgrowth
  • PNS
  • Pervin

ASJC Scopus subject areas

  • General Neuroscience


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