A P2X purinoceptor expressed by a subset of sensory neurons

Chih Cheng Chen, Armen N Akopian, Lucia Sivilotti, David Colquhoun, Geoffrey Burnstock, John N. Wood

Research output: Contribution to journalArticle

856 Citations (Scopus)

Abstract

ATP is known to depolarize sensory neurons, and may play a role in nociceptor activation when released from damaged tissue. Here we report the molecular cloning and characterization of a new member of the P2X receptor family, P2X3, expressed by these cells. The channel transcript was present in a subset of rat dorsal-root-ganglion sensory neurons, some of which express nociceptor-associated markers; it was absent in other tissues that were tested, including sympathetic, enteric and central nervous system neurons. Moreover, when expressed in Xenopus oocytes, the channel showed an ATP-dependent cation flux. P2X3 is the only ligand-gated channel known to be expressed exclusively by a subset of sensory neurons. The remarkable selectivity of expression of the channel coupled with its sensory neuron-like pharmacology suggests that this channel may transduce ATP-evoked nociceptor activation.

Original languageEnglish (US)
Pages (from-to)428-431
Number of pages4
JournalNature
Volume377
Issue number6548
DOIs
StatePublished - Oct 5 1995
Externally publishedYes

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Purinergic P2X Receptors
Sensory Receptor Cells
Nociceptors
Adenosine Triphosphate
Purinergic P2X3 Receptors
Ligand-Gated Ion Channels
Enteric Nervous System
Sympathetic Nervous System
Spinal Ganglia
Molecular Cloning
Xenopus
Oocytes
Cations
Central Nervous System
Pharmacology
Neurons

ASJC Scopus subject areas

  • General

Cite this

Chen, C. C., Akopian, A. N., Sivilotti, L., Colquhoun, D., Burnstock, G., & Wood, J. N. (1995). A P2X purinoceptor expressed by a subset of sensory neurons. Nature, 377(6548), 428-431. https://doi.org/10.1038/377428a0

A P2X purinoceptor expressed by a subset of sensory neurons. / Chen, Chih Cheng; Akopian, Armen N; Sivilotti, Lucia; Colquhoun, David; Burnstock, Geoffrey; Wood, John N.

In: Nature, Vol. 377, No. 6548, 05.10.1995, p. 428-431.

Research output: Contribution to journalArticle

Chen, CC, Akopian, AN, Sivilotti, L, Colquhoun, D, Burnstock, G & Wood, JN 1995, 'A P2X purinoceptor expressed by a subset of sensory neurons', Nature, vol. 377, no. 6548, pp. 428-431. https://doi.org/10.1038/377428a0
Chen CC, Akopian AN, Sivilotti L, Colquhoun D, Burnstock G, Wood JN. A P2X purinoceptor expressed by a subset of sensory neurons. Nature. 1995 Oct 5;377(6548):428-431. https://doi.org/10.1038/377428a0
Chen, Chih Cheng ; Akopian, Armen N ; Sivilotti, Lucia ; Colquhoun, David ; Burnstock, Geoffrey ; Wood, John N. / A P2X purinoceptor expressed by a subset of sensory neurons. In: Nature. 1995 ; Vol. 377, No. 6548. pp. 428-431.
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