A novel transcript is up-regulated by fasting in the hypothalamus and enhances insulin signalling

B. Chai, J. Y. Li, D. Fritze, W. Zhang, Z. Xia, M. W. Mulholland

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


A transcript of unknown function, regulated by fasting and feeding, was identified by microarray analysis. The transcript is up-regulated in the fasting state. An 1168-bp cDNA was cloned from rat hypothalamus and sequenced. This sequence is consistent with adipogenesis down-regulating transcript 3 (AGD3) (also known as human OCC-1) mRNA. A protein sequence identical to AGD3 was determined by mass spectrometry. In the rat brain, AGD3 mRNA is distributed in the arcuate nucleus, ventromedial hypothalamus, amygdaloid nuclei, hippocampus, and somatic cortex. Double in situ hybridisation showed that AGD3 mRNA is co-localised with pro-opiomelanocortin and neuropeptide Y in arcuate nucleus neurones. AGD3 binds with insulin receptor substrate 4 and increases insulin-stimulated phospho-Akt and regulates AMP-activated protein kinase and mammalian target of rapamycin downstream target S6 kinase phosphorylation.

Original languageEnglish (US)
Pages (from-to)292-301
Number of pages10
JournalJournal of Neuroendocrinology
Issue number3
StatePublished - Mar 2013
Externally publishedYes


  • Adipogenesis down-regulating transcript 3
  • Insulin
  • Neuropeptide Y
  • Proopiomelanocortin

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience


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