A novel transcript is up-regulated by fasting in the hypothalamus and enhances insulin signalling

B. Chai; J. Y. Li; D. Fritze; W. Zhang; Z. Xia; M. W. Mulholland

doi:10.1111/j.1365-2826.2012.02378.x

A novel transcript is up-regulated by fasting in the hypothalamus and enhances insulin signalling

B. Chai, J. Y. Li, D. Fritze, W. Zhang, Z. Xia, M. W. Mulholland

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A transcript of unknown function, regulated by fasting and feeding, was identified by microarray analysis. The transcript is up-regulated in the fasting state. An 1168-bp cDNA was cloned from rat hypothalamus and sequenced. This sequence is consistent with adipogenesis down-regulating transcript 3 (AGD3) (also known as human OCC-1) mRNA. A protein sequence identical to AGD3 was determined by mass spectrometry. In the rat brain, AGD3 mRNA is distributed in the arcuate nucleus, ventromedial hypothalamus, amygdaloid nuclei, hippocampus, and somatic cortex. Double in situ hybridisation showed that AGD3 mRNA is co-localised with pro-opiomelanocortin and neuropeptide Y in arcuate nucleus neurones. AGD3 binds with insulin receptor substrate 4 and increases insulin-stimulated phospho-Akt and regulates AMP-activated protein kinase and mammalian target of rapamycin downstream target S6 kinase phosphorylation.

Original language	English (US)
Pages (from-to)	292-301
Number of pages	10
Journal	Journal of Neuroendocrinology
Volume	25
Issue number	3
DOIs	https://doi.org/10.1111/j.1365-2826.2012.02378.x
State	Published - Mar 2013
Externally published	Yes

Keywords

Adipogenesis down-regulating transcript 3
Insulin
Neuropeptide Y
Proopiomelanocortin

ASJC Scopus subject areas

Endocrine and Autonomic Systems
Endocrinology
Cellular and Molecular Neuroscience
Endocrinology, Diabetes and Metabolism

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10.1111/j.1365-2826.2012.02378.x

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title = "A novel transcript is up-regulated by fasting in the hypothalamus and enhances insulin signalling",

abstract = "A transcript of unknown function, regulated by fasting and feeding, was identified by microarray analysis. The transcript is up-regulated in the fasting state. An 1168-bp cDNA was cloned from rat hypothalamus and sequenced. This sequence is consistent with adipogenesis down-regulating transcript 3 (AGD3) (also known as human OCC-1) mRNA. A protein sequence identical to AGD3 was determined by mass spectrometry. In the rat brain, AGD3 mRNA is distributed in the arcuate nucleus, ventromedial hypothalamus, amygdaloid nuclei, hippocampus, and somatic cortex. Double in situ hybridisation showed that AGD3 mRNA is co-localised with pro-opiomelanocortin and neuropeptide Y in arcuate nucleus neurones. AGD3 binds with insulin receptor substrate 4 and increases insulin-stimulated phospho-Akt and regulates AMP-activated protein kinase and mammalian target of rapamycin downstream target S6 kinase phosphorylation.",

keywords = "Adipogenesis down-regulating transcript 3, Insulin, Neuropeptide Y, Proopiomelanocortin",

author = "B. Chai and Li, {J. Y.} and D. Fritze and W. Zhang and Z. Xia and Mulholland, {M. W.}",

year = "2013",

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doi = "10.1111/j.1365-2826.2012.02378.x",

language = "English (US)",

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AU - Chai, B.

AU - Li, J. Y.

AU - Fritze, D.

AU - Zhang, W.

AU - Xia, Z.

AU - Mulholland, M. W.

PY - 2013/3

Y1 - 2013/3

N2 - A transcript of unknown function, regulated by fasting and feeding, was identified by microarray analysis. The transcript is up-regulated in the fasting state. An 1168-bp cDNA was cloned from rat hypothalamus and sequenced. This sequence is consistent with adipogenesis down-regulating transcript 3 (AGD3) (also known as human OCC-1) mRNA. A protein sequence identical to AGD3 was determined by mass spectrometry. In the rat brain, AGD3 mRNA is distributed in the arcuate nucleus, ventromedial hypothalamus, amygdaloid nuclei, hippocampus, and somatic cortex. Double in situ hybridisation showed that AGD3 mRNA is co-localised with pro-opiomelanocortin and neuropeptide Y in arcuate nucleus neurones. AGD3 binds with insulin receptor substrate 4 and increases insulin-stimulated phospho-Akt and regulates AMP-activated protein kinase and mammalian target of rapamycin downstream target S6 kinase phosphorylation.

AB - A transcript of unknown function, regulated by fasting and feeding, was identified by microarray analysis. The transcript is up-regulated in the fasting state. An 1168-bp cDNA was cloned from rat hypothalamus and sequenced. This sequence is consistent with adipogenesis down-regulating transcript 3 (AGD3) (also known as human OCC-1) mRNA. A protein sequence identical to AGD3 was determined by mass spectrometry. In the rat brain, AGD3 mRNA is distributed in the arcuate nucleus, ventromedial hypothalamus, amygdaloid nuclei, hippocampus, and somatic cortex. Double in situ hybridisation showed that AGD3 mRNA is co-localised with pro-opiomelanocortin and neuropeptide Y in arcuate nucleus neurones. AGD3 binds with insulin receptor substrate 4 and increases insulin-stimulated phospho-Akt and regulates AMP-activated protein kinase and mammalian target of rapamycin downstream target S6 kinase phosphorylation.

KW - Adipogenesis down-regulating transcript 3

KW - Insulin

KW - Neuropeptide Y

KW - Proopiomelanocortin

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A novel transcript is up-regulated by fasting in the hypothalamus and enhances insulin signalling

Abstract

Keywords

ASJC Scopus subject areas

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