A novel synaptobrevin/VAMP homologous protein (VAMP5) is increased during in vitro myogenesis and present in the plasma membrane

Qi Zeng, V. Nathan Subramaniam, Siew Heng Wong, Bor Luen Tang, Robert G. Parton, Shane Rea, David E. James, Wanjin Hong

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

cDNA clones encoding a novel protein (VAMP5) homologous to synaptobrevins/VAMPs are detected during database searches. The predicted 102-amino acid VAMP5 harbors a 23-residue hydrophobic region near the carboxyl terminus and exhibits an overall amino acid identity of 33% with synaptobrevin/VAMP1 and 2 and cellubrevin. Northern blot analysis reveals that the mRNA for VAMP5 is preferentially expressed in the skeletal muscle and heart, whereas significantly lower levels are detected in several other tissues but not in the brain. During in vitro differentiation (myogenesis) of C2C12 myoblasts into myotubes, the mRNA level for VAMP5 is increased ~8- to 10-fold. Immunoblot analysis using antibodies specific for VAMP5 shows that the protein levels are also elevated ~6-fold during in vitro myogenesis of C2C12 cells. Indirect immunofluorescence microscopy and immunoelectron microscopy reveal that VAMP5 is associated with the plasma membrane as well as intracellular perinuclear and peripheral vesicular structures of myotubes. Epitope-tagged versions of VAMP5 are similarly targeted to the plasma membrane.

Original languageEnglish (US)
Pages (from-to)2423-2437
Number of pages15
JournalMolecular Biology of the Cell
Volume9
Issue number9
DOIs
StatePublished - Sep 1998

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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