A novel regulatory element associated with age-dependent expression of the rat androgen receptor gene

P. C. Supakar, C. S. Song, M. H. Jung, M. A. Slomczynska, J. M. Kim, R. L. Vellanoweth, B. Chatterjee, A. K. Roy

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Abstract

A large body of evidence indicates that the genetic program of aging has co-evolved with the sexual mode of reproduction (Partridge, L., and Barton, N. H. (1993) Nature 362, 305-311). Age-dependent changes in target cell sensitivity to reproductive hormones can be considered part of this evolutionary linkage. Here we describe a novel regulatory element in the rat androgen receptor (AR) gene promoter associated with its age-dependent expression in the liver. This element consists of two (19 and 25 base pairs) contiguous sites, one specifically binding an Age-dependent Factor (ADF) and the other an Associated Factor (AF). Both deletion and point mutations of the ADF site result in about a 5-fold decline in the AR promoter function. Unlike AF, which is relatively tissue specific, ADF appears to be ubiquitous. The ubiquitous and evolutionarily conserved nature of ADF suggests a fundamental role of this novel transcription factor in programmed gene expression.

Original languageEnglish (US)
Pages (from-to)26400-26408
Number of pages9
JournalJournal of Biological Chemistry
Volume268
Issue number35
Publication statusPublished - Jan 1 1993

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Supakar, P. C., Song, C. S., Jung, M. H., Slomczynska, M. A., Kim, J. M., Vellanoweth, R. L., ... Roy, A. K. (1993). A novel regulatory element associated with age-dependent expression of the rat androgen receptor gene. Journal of Biological Chemistry, 268(35), 26400-26408.