A novel lipoprotein from Oomycete fungi

Steven A. Warner, David F. Eierman, Aristotle J. Domnas, David D. Dean

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

This work reports on the presence of an endogenous lipoprotein inLagenidium giganteum andPhytophthora cinnamomi as well as from the related non-sterol-deficient Oomycete speciesLagenidium callinectes, andZoophagus insidians. The lipoproteins comprise 0.2-1.0% of the total cell protein and are easily extracted with 0.2% Tween-80. The lipoprotein is homogeneous following heparin-agarose chromatography and nondenaturing gel electrophoresis. This holoprotein from all species had a molecular mass of 320,000 Da and contained four identical protein subunits of molecular mass 40,600. The lipoprotein exhibited similar physical characteristics to human low-density lipoprotein (LDL) as it comigrated with LDL on agarose gels, coeluted with LDL from heparin-agarose columns, and had a similar buoyant density of 1.006-1.063 g/cm3. The associated lipids varied quantitatively by species and included dolichol, diglycerides, and phospholipids. The lipoproteins from sterol-deficient species bound exogenous cholesterol without displacement of any of the native lipids of the lipoprotein.

Original languageEnglish (US)
Pages (from-to)315-322
Number of pages8
JournalExperimental Mycology
Volume10
Issue number4
DOIs
StatePublished - Dec 1986

Keywords

  • Oomycetes
  • composition
  • dolichol
  • isolation
  • lipoprotein
  • sterol

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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    Warner, S. A., Eierman, D. F., Domnas, A. J., & Dean, D. D. (1986). A novel lipoprotein from Oomycete fungi. Experimental Mycology, 10(4), 315-322. https://doi.org/10.1016/0147-5975(86)90018-6