A nosocomial outbreak of ampicillin-resistant Haemophilus influenzae type b in a geriatric unit

Jan E Patterson, G. M. Madden, E. P. Krisiunas, B. Masecar, W. J. Hierholzer, M. J. Zervos, R. W. Lyons

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A nosocomial outbreak of Haemophilus influenzae type b (Hib) bronchitis occurred in a geriatric unit. The three infected patients were grouped together in an isolation unit and treated. A prevalence survey was done by obtaining pharyngeal cultures from patients and staff in the unit. One patient and a nurse were asymptomatic pharyngeal carriers of Hib. One infected patient was bedridden, and his only known Hib contact was the nurse. Geographic clustering was the only significant risk factor, as determined by a case-control study. Carriers were treated with rifampin. The isolates were characterized for strain relatedness by using three methods. All produced β-lactamase and all were serotype b. Plasmid profiles and restriction endonuclease analysis of bacterial DNA were performed; chromosomes were digested with the restriction endonucleases HindIII and HaeIII. Strains were confirmed as identical by using these methods and were different from two Hib control strains producing β-lactamase. This study documents nosocomial transmission of Hib, by using molecular typing methods.

Original languageEnglish (US)
Pages (from-to)1002-1007
Number of pages6
JournalJournal of Infectious Diseases
Volume157
Issue number5
StatePublished - 1988
Externally publishedYes

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Haemophilus influenzae type b
Ampicillin
Geriatrics
Disease Outbreaks
DNA Restriction Enzymes
Deoxyribonuclease HindIII
Nurses
Molecular Typing
Bacterial DNA
Bronchitis
Rifampin
Cluster Analysis
Case-Control Studies
Plasmids
Chromosomes

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Patterson, J. E., Madden, G. M., Krisiunas, E. P., Masecar, B., Hierholzer, W. J., Zervos, M. J., & Lyons, R. W. (1988). A nosocomial outbreak of ampicillin-resistant Haemophilus influenzae type b in a geriatric unit. Journal of Infectious Diseases, 157(5), 1002-1007.

A nosocomial outbreak of ampicillin-resistant Haemophilus influenzae type b in a geriatric unit. / Patterson, Jan E; Madden, G. M.; Krisiunas, E. P.; Masecar, B.; Hierholzer, W. J.; Zervos, M. J.; Lyons, R. W.

In: Journal of Infectious Diseases, Vol. 157, No. 5, 1988, p. 1002-1007.

Research output: Contribution to journalArticle

Patterson, JE, Madden, GM, Krisiunas, EP, Masecar, B, Hierholzer, WJ, Zervos, MJ & Lyons, RW 1988, 'A nosocomial outbreak of ampicillin-resistant Haemophilus influenzae type b in a geriatric unit', Journal of Infectious Diseases, vol. 157, no. 5, pp. 1002-1007.
Patterson JE, Madden GM, Krisiunas EP, Masecar B, Hierholzer WJ, Zervos MJ et al. A nosocomial outbreak of ampicillin-resistant Haemophilus influenzae type b in a geriatric unit. Journal of Infectious Diseases. 1988;157(5):1002-1007.
Patterson, Jan E ; Madden, G. M. ; Krisiunas, E. P. ; Masecar, B. ; Hierholzer, W. J. ; Zervos, M. J. ; Lyons, R. W. / A nosocomial outbreak of ampicillin-resistant Haemophilus influenzae type b in a geriatric unit. In: Journal of Infectious Diseases. 1988 ; Vol. 157, No. 5. pp. 1002-1007.
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