A next-generation proteome array for Schistosoma mansoni

Rafael Ramiro de Assis, Fernanda Ludolf, Rie Nakajima, Al Jasinskas, Guilherme C. Oliveira, Philip L. Felgner, Soraya T. Gaze, Alex Loukas, Philip T. LoVerde, Jeffrey M. Bethony, Rodrigo Correa-Oliveira, Carlos E. Calzavara-Silva

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A proteome microarray consisting of 992 Schistosoma mansoni proteins was produced and screened with sera to determine antibody signatures indicative of the clinical stages of schistosomiasis and the identification of subunit vaccine candidates. Herein, we describe the methods used to derive the gene list for this array (representing approximately 10% of the predicted S. mansoni proteome). We also probed a pilot version of the microarray with sera from individuals either acutely or chronically infected with S. mansoni from endemic areas in Brazil and sera from individuals resident outside the endemic area (USA) to determine if the array is functional and informative.

Original languageEnglish (US)
Pages (from-to)411-415
Number of pages5
JournalInternational Journal for Parasitology
Volume46
Issue number7
DOIs
StatePublished - Jun 1 2016

Keywords

  • Antibody signature
  • Protein microarray
  • Proteome
  • Schistosoma mansoni

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'A next-generation proteome array for Schistosoma mansoni'. Together they form a unique fingerprint.

Cite this