A new mode of rotating gel electrophoresis for fractionating linear and circular duplex DNA: the effects of electrophoresis during the gel's rotation.

P. Serwer; S. J. Hayes

A new mode of rotating gel electrophoresis for fractionating linear and circular duplex DNA: the effects of electrophoresis during the gel's rotation.

P. Serwer, S. J. Hayes

Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Linear and circular DNA have been fractionated by use of rotating gel electrophoresis (RGE; RGE is a form of pulsed-field gel [PFG] electrophoresis) in a mode not previously described. This new mode consists of electrophoresis while repeating the following two-step process: rotating the gel by 2 pi radians, followed by holding the gel motionless. With this new mode of RGE: (1) The fractionation by length of linear DNA is greater than that achieved without changing the direction of the field. (2) The simultaneous separation of open circular DNA (48.5-97 Kb) from linear DNA (1-180 Kb) and fractionation by length of open circular DNA is achieved. These latter separations have not previously been achieved by a one-dimensional electrophoresis. The mechanism of linear DNA's sieving in the new mode appears different from that of all previously described procedures of PGF electrophoresis.

Original language	English (US)
Pages (from-to)	95-98
Number of pages	4
Journal	Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society
Volume	1
Issue number	2
State	Published - 1989

ASJC Scopus subject areas

Medicine(all)

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A new mode of rotating gel electrophoresis for fractionating linear and circular duplex DNA : the effects of electrophoresis during the gel's rotation. / Serwer, P.; Hayes, S. J.

In: Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society, Vol. 1, No. 2, 1989, p. 95-98.

Research output: Contribution to journal › Article › peer-review

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