Abstract
Linear and circular DNA have been fractionated by use of rotating gel electrophoresis (RGE; RGE is a form of pulsed-field gel [PFG] electrophoresis) in a mode not previously described. This new mode consists of electrophoresis while repeating the following two-step process: rotating the gel by 2 pi radians, followed by holding the gel motionless. With this new mode of RGE: (1) The fractionation by length of linear DNA is greater than that achieved without changing the direction of the field. (2) The simultaneous separation of open circular DNA (48.5-97 Kb) from linear DNA (1-180 Kb) and fractionation by length of open circular DNA is achieved. These latter separations have not previously been achieved by a one-dimensional electrophoresis. The mechanism of linear DNA's sieving in the new mode appears different from that of all previously described procedures of PGF electrophoresis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 95-98 |
| Number of pages | 4 |
| Journal | Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society |
| Volume | 1 |
| Issue number | 2 |
| State | Published - 1989 |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
A new mode of rotating gel electrophoresis for fractionating linear and circular duplex DNA : the effects of electrophoresis during the gel's rotation. / Serwer, Philip; Hayes, S. J.
In: Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society, Vol. 1, No. 2, 1989, p. 95-98.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A new mode of rotating gel electrophoresis for fractionating linear and circular duplex DNA
T2 - the effects of electrophoresis during the gel's rotation.
AU - Serwer, Philip
AU - Hayes, S. J.
PY - 1989
Y1 - 1989
N2 - Linear and circular DNA have been fractionated by use of rotating gel electrophoresis (RGE; RGE is a form of pulsed-field gel [PFG] electrophoresis) in a mode not previously described. This new mode consists of electrophoresis while repeating the following two-step process: rotating the gel by 2 pi radians, followed by holding the gel motionless. With this new mode of RGE: (1) The fractionation by length of linear DNA is greater than that achieved without changing the direction of the field. (2) The simultaneous separation of open circular DNA (48.5-97 Kb) from linear DNA (1-180 Kb) and fractionation by length of open circular DNA is achieved. These latter separations have not previously been achieved by a one-dimensional electrophoresis. The mechanism of linear DNA's sieving in the new mode appears different from that of all previously described procedures of PGF electrophoresis.
AB - Linear and circular DNA have been fractionated by use of rotating gel electrophoresis (RGE; RGE is a form of pulsed-field gel [PFG] electrophoresis) in a mode not previously described. This new mode consists of electrophoresis while repeating the following two-step process: rotating the gel by 2 pi radians, followed by holding the gel motionless. With this new mode of RGE: (1) The fractionation by length of linear DNA is greater than that achieved without changing the direction of the field. (2) The simultaneous separation of open circular DNA (48.5-97 Kb) from linear DNA (1-180 Kb) and fractionation by length of open circular DNA is achieved. These latter separations have not previously been achieved by a one-dimensional electrophoresis. The mechanism of linear DNA's sieving in the new mode appears different from that of all previously described procedures of PGF electrophoresis.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024893501&partnerID=8YFLogxK
M3 - Article
C2 - 2535116
AN - SCOPUS:0024893501
VL - 1
SP - 95
EP - 98
JO - Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society
JF - Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society
SN - 0954-6642
IS - 2
ER -