A new medium for primary cultures of adult murine epidermal cells: Application to experimental carcinogenesis

Rebecca J. Morris, Kay C. Tacker, James K. Baldwin, Susan M. Fischer, Thomas J. Slaga

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

A culture medium has been formulated to support the long-term proliferation and differentiation of normal murine epidermal cells in primary cultures. This formulation is based upon MCDB-151 modified by the addition of 1.2 mM Ca2+, 1% fetal bovine serum (FBS) or 0.1% purified bovine serum albumin (BSA), trace elements, and growth supplements. For 4-12 weeks, the cultures demonstrate desmosomes, keratin fibers, basal proliferation, and stratification characteristic of keratinocytes. The addition of 12-O-tetradecanoylphorbol-13-acetate (TPA) resulted in approximately 70% cellular detachment, but with continued treatment, the remaining cells proliferated to confluence and stratified. This culture medium has application to a number of studies related to skin carcinogenesis in the murine model system that have not previously been possible.

Original languageEnglish (US)
Pages (from-to)297-304
Number of pages8
JournalCancer Letters
Volume34
Issue number3
DOIs
Publication statusPublished - Mar 1987

    Fingerprint

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this