A new DNA marker, U15557, is linked to protease inhibitor and adenylate kinase-1 in the laboratory opossum, Monodelphis domestica

A. A. Perelygin, P. B. Samollow, L. M. Perelygina, L. M. Cherry, S. M. Mahaney, J. L. VandeBerg

    Research output: Contribution to journalArticlepeer-review

    6 Scopus citations

    Abstract

    A 323-bp DNA fragment (U15557) was isolated, cloned, and sequenced after polymerase chain reaction (PCR) amplification from Monodelphis domestica genomic DNA. A HindIII restriction fragment length polymorphism was identified in this species using the U15557 PCR. fragment as a hybridization probe. DNA samples exhibited either a 6·4 kb band, a 7·2 kb band, or both bands simultaneously. Behaviour of these two variants in family studies was consistent with codominant autosomal inheritance. Linkage between this marker and the loci encoding protease inhibitor (PI) and adenylate kinase 1 (AK1) was found in M. domestica.

    Original languageEnglish (US)
    Pages (from-to)113-116
    Number of pages4
    JournalAnimal genetics
    Volume27
    Issue number2
    DOIs
    StatePublished - Apr 1996

    Keywords

    • Laboratory opossum
    • Linkage
    • Sex-specific recombination rates

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • Genetics

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