A mouse model of orthotopic vascularized aerated lung transplantation

M. Okazaki, A. S. Krupnick, C. G. Kornfeld, J. M. Lai, J. H. Ritter, S. B. Richardson, H. J. Huang, N. A. Das, G. A. Patterson, A. E. Gelman, D. Kreisel

Research output: Contribution to journalArticlepeer-review

115 Scopus citations

Abstract

Outcomes after lung transplantation are markedly inferior to those after other solid organ transplants. A better understanding of cellular and molecular mechanisms contributing to lung graft injury will be critical to improve outcomes. Advances in this field have been hampered by the lack of a mouse model of lung transplantation. Here, we report a mouse model of vascularized aerated single lung transplantation utilizing cuff techniques. We show that syngeneic grafts have normal histological appearance with minimal infiltration of T lymphocytes. Allogeneic grafts show acute cellular rejection with infiltration of T lymphocytes and recipient-type antigen presenting cells. Our data show that we have developed a physiological model of lung transplantation in the mouse, which provides ample opportunity for the study of nonimmune and immune mechanisms that contribute to lung allograft injury.

Original languageEnglish (US)
Pages (from-to)1672-1679
Number of pages8
JournalAmerican Journal of Transplantation
Volume7
Issue number6
DOIs
StatePublished - Jun 2007

Keywords

  • Lung transplantation
  • Mice
  • Surgical technique

ASJC Scopus subject areas

  • Immunology and Allergy
  • Transplantation
  • Pharmacology (medical)

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