TY - CHAP
T1 - A molecular overview of diapause in embryos of the crustacean, Artemia franciscana
AU - Qiu, Zhijun
AU - MacRae, Thomas H.
N1 - Funding Information:
Research described in this chapter was supported by a Natural Sciences and Engineering Research Council of Canada Discovery Grant to T.H.M.
PY - 2010
Y1 - 2010
N2 - Diapause-destined embryos of Artemia franciscana arrest as gastrulae and are enclosed within a chitinous shell to form cysts. Upon release from females encysted embryos enter diapause, becoming essentially ametabolic and very stress tolerant. An objective of our research is to characterize the molecular mechanisms of diapause initiation and maintenance. Subtractive hybridization was therefore used to identify up-regulated genes in diapause-destined Artemia embryos and these were divided into functional categories including cellular growth and stress tolerance, metabolism, and genetic and environmental information processing. Of particular interest mRNA for p8, a stress-inducible transcription co-factor, increased early in diapause-destined Artemia embryos, representing one of the few transcription co-factors known to be up-regulated during diapause. mRNAs encoding the three small heat shock proteins p26, ArHsp21, and ArHsp22 were also up-regulated and these proteins may promote diapause maintenance by enhancing stress tolerance. The work has revealed proteins potentially crucial to diapause, a physiological condition of fundamental and applied significance.
AB - Diapause-destined embryos of Artemia franciscana arrest as gastrulae and are enclosed within a chitinous shell to form cysts. Upon release from females encysted embryos enter diapause, becoming essentially ametabolic and very stress tolerant. An objective of our research is to characterize the molecular mechanisms of diapause initiation and maintenance. Subtractive hybridization was therefore used to identify up-regulated genes in diapause-destined Artemia embryos and these were divided into functional categories including cellular growth and stress tolerance, metabolism, and genetic and environmental information processing. Of particular interest mRNA for p8, a stress-inducible transcription co-factor, increased early in diapause-destined Artemia embryos, representing one of the few transcription co-factors known to be up-regulated during diapause. mRNAs encoding the three small heat shock proteins p26, ArHsp21, and ArHsp22 were also up-regulated and these proteins may promote diapause maintenance by enhancing stress tolerance. The work has revealed proteins potentially crucial to diapause, a physiological condition of fundamental and applied significance.
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U2 - 10.1007/978-3-642-12422-8_10
DO - 10.1007/978-3-642-12422-8_10
M3 - Chapter
AN - SCOPUS:77955965275
SN - 9783642124211
T3 - Topics in Current Genetics
SP - 165
EP - 187
BT - Dormancy and Resistance in Harsh Environments
A2 - Lubzens, Esther
ER -