A microsatellite-based, physically anchored linkage map for the gray, short-tailed Opossum (Monodelphis domestica)

Paul B. Samollow, Nicolas Gouin, Pat Miethke, Susan M. Mahaney, Margaret Kenney, John L. VandeBerg, Jennifer A Marshall Graves, Candace M. Kammerer

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The genome of the gray, short-tailed opossum, Monodelphis domestica, will be the first of any marsupial to be fully sequenced. The utility of this sequence will be greatly enhanced by construction and integration of detailed genetic and physical maps. Therefore, it is important to verify the unusual recombinational characteristics that were suggested by the 'first-generation' M. domestica linkage map; specifically, very low levels of recombination and severely reduced female recombination, both of which are contrary to patterns in other vertebrates. We constructed a new linkage map based on a different genetic cross, using a new and much larger set of map markers, and physically anchored and oriented the linkage groups onto chromosomes via fluorescence in-situ hybridization mapping. This map includes 150 loci in eight autosomal linkage groups corresponding to the eight autosome pairs, and spans 86-89% of the autosomal genome. The sex-averaged autosomal map covers 715 cM, with a full-length estimate of 866 cM; the shortest full-length linkage map reported for any vertebrate. The sex-specific maps confirmed severely reduced female recombination in all linkage groups, and an overall F/M map ratio = 0.54. These results greatly extend earlier findings, and provide an improved microsatellite-based linkage map for this species.

Original languageEnglish (US)
Pages (from-to)269-281
Number of pages13
JournalChromosome Research
Volume15
Issue number3
DOIs
StatePublished - Apr 2007
Externally publishedYes

Fingerprint

Monodelphis
Microsatellite Repeats
Genetic Recombination
Vertebrates
Genetic Crosses
Genome
Marsupialia
Fluorescence In Situ Hybridization
Chromosomes

Keywords

  • Linkage
  • Monodelphis domestica
  • Physical mapping
  • Recombination
  • Sex-specific

ASJC Scopus subject areas

  • Genetics

Cite this

Samollow, P. B., Gouin, N., Miethke, P., Mahaney, S. M., Kenney, M., VandeBerg, J. L., ... Kammerer, C. M. (2007). A microsatellite-based, physically anchored linkage map for the gray, short-tailed Opossum (Monodelphis domestica). Chromosome Research, 15(3), 269-281. https://doi.org/10.1007/s10577-007-1123-4

A microsatellite-based, physically anchored linkage map for the gray, short-tailed Opossum (Monodelphis domestica). / Samollow, Paul B.; Gouin, Nicolas; Miethke, Pat; Mahaney, Susan M.; Kenney, Margaret; VandeBerg, John L.; Graves, Jennifer A Marshall; Kammerer, Candace M.

In: Chromosome Research, Vol. 15, No. 3, 04.2007, p. 269-281.

Research output: Contribution to journalArticle

Samollow, PB, Gouin, N, Miethke, P, Mahaney, SM, Kenney, M, VandeBerg, JL, Graves, JAM & Kammerer, CM 2007, 'A microsatellite-based, physically anchored linkage map for the gray, short-tailed Opossum (Monodelphis domestica)', Chromosome Research, vol. 15, no. 3, pp. 269-281. https://doi.org/10.1007/s10577-007-1123-4
Samollow, Paul B. ; Gouin, Nicolas ; Miethke, Pat ; Mahaney, Susan M. ; Kenney, Margaret ; VandeBerg, John L. ; Graves, Jennifer A Marshall ; Kammerer, Candace M. / A microsatellite-based, physically anchored linkage map for the gray, short-tailed Opossum (Monodelphis domestica). In: Chromosome Research. 2007 ; Vol. 15, No. 3. pp. 269-281.
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