A method for rapid filling of fine-tipped micropipettes with electrolyte solutions including those containing horseradish peroxidase

Lewis B. Haberly, James M. Bower

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Cooling the tips of micropipettes pulled from filament-containing capillary glass dramatically increases the rate and completeness of filling with a variety of electrolyte solutions. In tests with solutions containing horseradish peroxidase this method increased the completeness of filling by more than two orders of magnitude.

Original languageEnglish (US)
Pages (from-to)251-254
Number of pages4
JournalJournal of Neuroscience Methods
Volume3
Issue number3
DOIs
StatePublished - Feb 1981

Keywords

  • horseradish peroxidase
  • intracellular injection
  • intracellular recording
  • iontophoresis
  • micropipette
  • neurophysiology

ASJC Scopus subject areas

  • Neuroscience(all)

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