A method for measurement of prolactin in the hamster by means of radioimmunoassay

R. J. Donofrio; R. J. Reiter; S. Sorrentino; D. E. Blask; Judy A. Talbot

doi:10.1159/000122199

A method for measurement of prolactin in the hamster by means of radioimmunoassay

R. J. Donofrio, R. J. Reiter, S. Sorrentino, D. E. Blask, Judy A. Talbot

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Pituitary and plasma prolactin levels have been measured in the golden hamster (Mesocricetus auratus) by means of a double antibody radioimmunoassay (RIA) using the NIAMD rat prolactin kit. The variance between the rat prolactin standard curve and the hamster pituitary dilution curve meant that all experimental data in the hamster had to be compared to a preparation of standard hamster anterior pituitary tissue (SHAP). Pituitary and plasma SHAP values in the hamster have been shown to fluctuate in a manner completely analogous to reported prolactin changes in other mammals, under the following conditions: Stress, lactation, inhibition of pituitary release in vitro by hypothalamic extract, and pineal activation. It is concluded that this system can accurately estimate prolactin levels in the hamster in both pituitary and plasma samples.

Original language	English (US)
Pages (from-to)	79-92
Number of pages	14
Journal	Neuroendocrinology
Volume	13
Issue number	1-2
DOIs	https://doi.org/10.1159/000122199
State	Published - Jan 1 1973
Externally published	Yes

Keywords

Hamster
Prolactin
Radioimmunoassay

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology
Endocrine and Autonomic Systems
Cellular and Molecular Neuroscience

Access to Document

10.1159/000122199

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abstract = "Pituitary and plasma prolactin levels have been measured in the golden hamster (Mesocricetus auratus) by means of a double antibody radioimmunoassay (RIA) using the NIAMD rat prolactin kit. The variance between the rat prolactin standard curve and the hamster pituitary dilution curve meant that all experimental data in the hamster had to be compared to a preparation of standard hamster anterior pituitary tissue (SHAP). Pituitary and plasma SHAP values in the hamster have been shown to fluctuate in a manner completely analogous to reported prolactin changes in other mammals, under the following conditions: Stress, lactation, inhibition of pituitary release in vitro by hypothalamic extract, and pineal activation. It is concluded that this system can accurately estimate prolactin levels in the hamster in both pituitary and plasma samples.",

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